MA5-12333

antibody from Invitrogen Antibodies

Targeting: MCM2 BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030

Antibody data

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Validation data

Product number: MA5-12333 - Provider product page
Provider: Invitrogen Antibodies
Product name: MCM2 Monoclonal Antibody (CRCT2.1)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-12333 targets MCM2 in IHC (P) and WB applications and shows reactivity with Human samples.
Antibody clone number: CRCT2.1
Concentration: 0.4 mg/mL

MCM2 protein structure - MA5-12333 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of MCM2 using MCM2 Monoclonal Antibody (Product # MA5-12333) on HeLa Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of MCM2 using MCM2 Monoclonal Antibody (Product # MA5-12333) on HeLa Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of MCM2 was achieved by transfecting MCF7 cells with MCM2 specific siRNA (Silencer® select Product # s8586 and s8587). Western blot analysis (Fig a) was performed using modified whole cell extract (1% SDS) from the MCM2 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-MCM2 Mouse monoclonal antibody (Product # MA5-12333, 2 µg/mL) and Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to MCM2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), A549 (Lane3), K-562 (Lane 4), Jurkat (Lane 5), MOLT4 (Lane 6) and HCT116 (Lane 7). The blot was probed with Mouse anti-MCM2 Monoclonal Antibody (Product # MA5-12333, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 125 kDa band corresponding to MCM2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).