NBP2-61939
antibody from Novus Biologicals
Targeting: CYP19A1
ARO, ARO1, aromatase, CPV1, CYAR, CYP19, P-450AROM
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-61939 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Aromatase Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aromatase Antibody ([H4]) - Azide and BSA Free [NBP2-61939] - Binding of H4 to a 55 kDa protein on the Cytochrome P450 aromatase protein by Western Blot, On Western blots, a single protein of 55 kDa was recognized by the monoclonal antibody raised against a peptide within the P450 aromatase protein (A). No signal was seen on blots incubated with antibody that had been pre-absorbed with immunizing peptide (B). Extracts were prepared from the following tissues: lane 1, human placenta; lane 2, marmoset placenta; lane 3, marmoset ovary; lane 4, ovary from pregnant rat; lanes 5 and 6, ovaries from two rats killed at pro-estrus.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Aromatase Antibody ([H4]) - Azide and BSA Free [NBP2-61939] - Clone H4 used to detect aromatase in human tissues by IHC-P. A: Intense aromatase immunostaining in IGCN cells. B: Placental-like alkaline phosphatase staining of IGCN basal cells C: Strong aromatase immunoreactivity of interstitial Leydig cells in normal testis (Lc) D: Intense immunostaining of luteal cells (Luc) in ovarian tissue.