AB-245-NA

antibody from Novus Biologicals

Targeting: TGFB1 CED, DPD1, TGFB, TGFbeta

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-245-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal TGF-beta 5 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects TGF-beta 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with the other TGF-beta variants is observed. Neutralizes the biological activity of recombinant amphibian TGF-beta 5, but not TGF-beta 1, porcine TGF-beta 1.2, TGF-beta 2, or TGF-beta 3.

Host

Goat

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

TGF-beta 5 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 5 Antibody. Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Amphibian TGF-beta 5 (0.1 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-TGF-beta 5 Polyclonal Antibody (Catalog # AB-245-NA). The ND₅₀ is typically 0.03-0.08 µg/mL.