IC1835N

antibody from Novus Biologicals

Targeting: TGFB1 CED, DPD1, TGFB, TGFbeta

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC1835N

Provider

Novus Biologicals

Product name

Mouse Monoclonal TGF beta 1/2/3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects bovine, chicken, mouse, and human TGF-beta in ELISAs and Western blots. It recognizes human TGF-beta 1, TGF-beta 2, and TGF-beta 3.

Host

Mouse

Conjugate

Near infrared dye

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of TGF-beta 1, 2, 3 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-TGF-beta 1, 2, 3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1835N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.