GTX110630

antibody from GeneTex

Targeting: TGFB1 CED, DPD1, TGFB, TGFbeta

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX110630

Provider

GeneTex

Proper citation

GeneTex Cat#GTX110630, RRID:AB_1952223

Product name

TGF beta 1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References

Submitted references

Retardation of Preputial Wound Healing in Rats with Hypospadias Induced by Flutamide.

Zhou Y, Peng J, Cao X, Yan C, Huang F, Shen L, Long C, Liu X, Wei G
Journal of investigative surgery : the official journal of the Academy of Surgical Research 2018 Oct 31;:1-8

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Gpr110 deficiency decelerates carcinogen-induced hepatocarcinogenesis via activation of the IL-6/STAT3 pathway.

Ma B, Zhu J, Tan J, Mao Y, Tang L, Shen C, Zhang H, Kuang Y, Fei J, Yang X, Wang Z
American journal of cancer research 2017;7(3):433-447

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QiShenYiQi pill attenuates atherosclerosis by promoting regulatory T cells, inhibiting T helper 17 cells and accelerating cholesterol excretion.

Peng L, Lv CS, Zhao Y, Chen SD, Huang Y, Lu DW, Huang SQ, Yang ZB, Qian LC, Wen L
Oncotarget 2017 Oct 10;8(47):82196-82206

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Hydrogen-containing saline alleviates pressure overload-induced interstitial fibrosis and cardiac dysfunction in rats.

Yang J, Wu S, Zhu L, Cai J, Fu L
Molecular medicine reports 2017 Aug;16(2):1771-1778

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Low-Level Light Therapy with 410 nm Light Emitting Diode Suppresses Collagen Synthesis in Human Keloid Fibroblasts: An In Vitro Study.

Lee HS, Jung SE, Kim SK, Kim YS, Sohn S, Kim YC
Annals of dermatology 2017 Apr;29(2):149-155

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The Role of Butylidenephthalide in Targeting the Microenvironment Which Contributes to Liver Fibrosis Amelioration.

Chuang HM, Su HL, Li C, Lin SZ, Yen SY, Huang MH, Ho LI, Chiou TW, Harn HJ
Frontiers in pharmacology 2016;7:112

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Cordyceps militaris Treatment Preserves Renal Function in Type 2 Diabetic Nephropathy Mice.

Yu SH, Dubey NK, Li WS, Liu MC, Chiang HS, Leu SJ, Shieh YH, Tsai FC, Deng WP
PloS one 2016;11(11):e0166342

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Novel hydrogen sulfide-releasing compound, S-propargyl-cysteine, prevents STZ-induced diabetic nephropathy.

Qian X, Li X, Ma F, Luo S, Ge R, Zhu Y
Biochemical and biophysical research communications 2016 May 13;473(4):931-938

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Fasudil, an inhibitor of Rho-associated coiled-coil kinase, attenuates hyperoxia-induced pulmonary fibrosis in neonatal rats.

Qi XJ, Ning W, Xu F, Dang HX, Fang F, Li J
International journal of clinical and experimental pathology 2015;8(10):12140-50

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Hsp70 Knockdown by siRNA Decreased Collagen Production in Keloid Fibroblasts.

Shin JU, Lee WJ, Tran TN, Jung I, Lee JH
Yonsei medical journal 2015 Nov;56(6):1619-26

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Proteomic profiling reveals upregulated protein expression of hsp70 in keloids.

Lee JH, Shin JU, Jung I, Lee H, Rah DK, Jung JY, Lee WJ
BioMed research international 2013;2013:621538

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Saikosaponin a and saikosaponin d inhibit proliferation and migratory activity of rat HSC-T6 cells.

Chen MF, Huang CC, Liu PS, Chen CH, Shiu LY
Journal of medicinal food 2013 Sep;16(9):793-800

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Thalidomide inhibits fibronectin production in TGF-β1-treated normal and keloid fibroblasts via inhibition of the p38/Smad3 pathway

Liang C, Yen Y, Hung L, Wang S, Pu C, Chien H, Tsai J, Lee C, Yen F, Chen Y
Biochemical Pharmacology 2013 June;85(11):1594-1602

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TGF beta 1 antibody detects TGFB1 protein by Western blot analysis.A. 30 µg Raji whole cell lysate/extract B. 30 µg K562 whole cell lysate/extract 10 % SDS-PAGETGF beta 1 antibody (GTX110630) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 μg of whole cell lysate) A: mouse spleen 10% SDS PAGE GTX110630 diluted at 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX110630) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX110630) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TGF beta 1 antibody detects TGF beta 1 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX110630) diluted by 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TGF beta 1(GTX110630) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TGF beta 1 antibody detects TGF beta 1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. TGF beta 1 antibody (GTX110630) dilution: 1:500.