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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AB-246-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human LAP (TGF-beta 1) Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human LAP in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody is specific for the LAP derived from the TGF-beta 1 precursor but does not react with LAP from the TGF-beta 2 precursor.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01137- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment.
Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-β1 processing.
Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis.
Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells.
Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen GH, Blakely EA, Bissell MJ, Barcellos-Hoff MH, Snijders AM, Mao JH
Scientific reports 2015 Mar 9;5:8919
Scientific reports 2015 Mar 9;5:8919
Knockdown of prolyl-4-hydroxylase domain 2 inhibits tumor growth of human breast cancer MDA-MB-231 cells by affecting TGF-β1 processing.
Wottawa M, Leisering P, Ahlen Mv, Schnelle M, Vogel S, Malz C, Bordoli MR, Camenisch G, Hesse A, Napp J, Alves F, Kristiansen G, Farhat K, Katschinski DM
International journal of cancer 2013 Jun 15;132(12):2787-98
International journal of cancer 2013 Jun 15;132(12):2787-98
Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis.
Lauzier A, Charbonneau M, Paquette M, Harper K, Dubois CM
Arthritis research & therapy 2012 Jul 4;14(4):R159
Arthritis research & therapy 2012 Jul 4;14(4):R159
Tumor metastasis in an orthotopic murine model of head and neck cancer: possible role of TGF-beta 1 secreted by the tumor cells.
Dasgupta S, Bhattacharya-Chatterjee M, O'Malley BW Jr, Chatterjee SK
Journal of cellular biochemistry 2006 Apr 1;97(5):1036-51
Journal of cellular biochemistry 2006 Apr 1;97(5):1036-51
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TGF-beta 1 in Human PBMCs. TGF-beta 1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue).View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LAP (TGF-beta 1) in Human Prostate Cancer Tissue. LAP (TGF-beta 1) was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human LAP (TGF-beta 1) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-246-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LAP TGF-beta 1 Inhibition of TGF-beta 1 Activity and Neutralization by Human LAP TGF-beta 1 Antibody. Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (0.25 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (125 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human LAP TGF-beta 1 Polyclonal Antibody (Catalog # AB-246-NA). The ND50 is typically 15-30 µg/mL.
