41-4800

antibody from Invitrogen Antibodies

Targeting: CTSB

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

41-4800

Provider

Invitrogen Antibodies

Product name

Cathepsin B Monoclonal Antibody (4B11)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4B11

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Irradiation of pediatric glioblastoma cells promotes radioresistance and enhances glioma malignancy via genome-wide transcriptome changes.

Alhajala HS, Nguyen HS, Shabani S, Best B, Kaushal M, Al-Gizawiy MM, Erin Ahn EY, Knipstein JA, Mirza S, Schmainda KM, Chitambar CR, Doan NB
Oncotarget 2018 Sep 25;9(75):34122-34131

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The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.

Marwaha R, Arya SB, Jagga D, Kaur H, Tuli A, Sharma M
The Journal of cell biology 2017 Apr 3;216(4):1051-1070

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Contact of high-invasive, but not low-invasive, melanoma cells to native collagen I induces the release of mature cathepsin B.

Klose A, Wilbrand-Hennes A, Zigrino P, Weber E, Krieg T, Mauch C, Hunzelmann N
International journal of cancer 2006 Jun 1;118(11):2735-43

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7. Binding to Arl8b is necessary for PLEKHM1 function in regulating endocytic cargo trafficking to lysosomes. (a) Schematic illustrating the uptake and further processing of DQ-BSA, an endocytic cargo in the cells. (b-e) Representative confocal images of HeLa cells treated with indicated siRNAs and subjected to DQ-BSA uptake for 6 h. The cells were then fixed and analyzed for DQ-BSA fluorescence. (f) Measurement of fold change in the fluorescence intensity of DQ-BSA from 1h to 6 h ( n = 3; 50 cells analyzed per experiment). (g-j) Representative confocal micrographs of HeLa cells treated with the indicated siRNAs and transfected with either siRNA-resistant PLEKHM1 (WT) or siRNA-resistant PLEKHM1 (HRR-A) construct and subjected to DQ-BSA uptake for 6 h. (k) Quantification of DQ-BSA trafficking in HeLa cells treated with indicated siRNAs and transfected with either siRNA-resistant PLEKHM1 (WT) or siRNA-resistant PLEKHM1 (HRR-A) construct ( n = 3; 50 cells analyzed per experiment). (l) Western blot of mature cathepsin B and D levels in control- or PLEKHM1-siRNA-treated HeLa cells. (m) PC was measured for cathepsin D, and LAMP1 colocalization in control- or PLEKHM1-siRNA-treated HeLa cells ( n = 3; 30 cells per experiment). (n) HeLa cells treated with control- or PLEKHM1-siRNA were incubated for 1 h in growth medium supplemented with cathepsin L substrate, and fluorescence intensity was measured by flow cytometry ( n = 3; 10,000 cells analyzed per experiment). Data represen