Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-110828 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cystatin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QVRSQLEEKE NKKFPVFKAV SFKSQVVAGT NYFIKVHVGD EDFVHLRVFQ SLPHENKPLT LSNYQTNKAK HDELT
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Cystatin B in human cell lines A-431 and U-251MG using Cystatin B Polyclonal Antibody (Product # PA5-110828). Corresponding Cystatin B RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Cystatin B in human cell line A-431 using Cystatin B Polyclonal Antibody (Product # PA5-110828) shows localization to cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Cystatin B in human esophagus and skeletal muscle tissues using Cystatin B Polyclonal Antibody (Product # PA5-110828). Corresponding Cystatin B RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Cystatin B in human liver tissue using Cystatin B Polyclonal Antibody (Product # PA5-110828). Staining shows no positivity in hepatocytes.as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Cystatin B in human tonsil tissue using Cystatin B Polyclonal Antibody (Product # PA5-110828). Staining shows moderate to strong nuclear and cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Cystatin B in human esophagus tissue using Cystatin B Polyclonal Antibody (Product # PA5-110828). Staining shows moderate to strong nuclear positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Cystatin B in human skeletal muscle tissue using Cystatin B Polyclonal Antibody (Product # PA5-110828). Staining shows no positivity in myocytes as expected.