NBP1-85430

antibody from Novus Biologicals

Targeting: CSTB CST6, EPM1, PME, STFB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-85430

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-85430, RRID:AB_11035006

Product name

Rabbit Polyclonal Cystatin B/Stefin B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Cystatin B/Stefin B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cystatin B/Stefin B Antibody [NBP1-85430] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-557

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cystatin B/Stefin B Antibody [NBP1-85430] - Analysis in human cell lines A-431 and U-251MG using anti-CSTB antibody. Corresponding CSTB RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human urinary bladder shows strong positivity in urothelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human esophagus shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human stomach shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Analysis in human esophagus and skeletal muscle tissues. Corresponding Cystatin B/Stefin B RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human esophagus shows moderate to strong nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human liver shows no positivity in hepatocytes.as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Cystatin B/Stefin B Antibody [NBP1-85430] - Staining of human tonsil shows moderate to strong nuclear and cytoplasmic positivity in squamous epithelial cells.