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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB100-722 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-722, RRID:AB_10000804
- Product name
- Rabbit Polyclonal Connexin 43/GJA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~43k connexin43 protein phosphorylated at Ser368. Immunolabeling is blocked by lambda-phosphatase treatment.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Connexin 43/GJA1 [p Ser368] Antibody [NB100-722] - rat hippocampal lysate showing specific immunolabeling of the ~43k connexin43 phosphorylated at Ser368 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the phospho-Ser368 connexin43 antibody. The immunolabeling of connexin43 is completely eliminated by treatment.
