TA503110

antibody from OriGene

Targeting: TUBB4A beta-5, DYT4, TUBB4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503110

Provider

OriGene

Proper citation

OriGene Cat#TA503110, RRID:AB_11124130

Product name

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI8E3 (formerly 8E3)

Antibody type

Monoclonal

Description

TUBB4 (beta IV Tubulin) mouse monoclonal antibody, clone OTI8E3 (formerly 8E3)

Host

Mouse

Conjugate

Unconjugated

Epitope

TUBB4A

Isotype

IgG

Antibody clone number

OTI8E3

Vial size

100 µl

Concentration

0.49 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB4 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB4 (RC203945, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB4.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-TUBB4 mouse monoclonal antibody (TA503110) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBB4(RC203945).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TUBB4 mouse monoclonal antibody. (TA503110)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-TUBB4 antibody(TA503110),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-TUBB4 antibody(TA503110),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Validation comment

FC