MIH9822

antibody from Invitrogen Antibodies

Targeting: CGB8

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MIH9822

Provider

Invitrogen Antibodies

Product name

hCG Monoclonal Antibody (34P2C2)

Antibody type

Monoclonal

Antigen

Other

Description

The observed molecular weight of alpha-hCG and beta-hCG are approximately 20-22 kD & 27-32 kD respectively.

Antibody clone number

34P2C2

Concentration

5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of hCG was performed by loading the indicated amounts of purified hCG protein extracted from human urine, and 15 µL of PageRuler Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~22 kD using a anti hCG antibody (Product # MIH9822) at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody (Product # 31439) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).