MIH9827

antibody from Invitrogen Antibodies

Targeting: CGB3 CGB

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MIH9827

Provider

Invitrogen Antibodies

Product name

hCG beta Monoclonal Antibody (P1G7F12)

Antibody type

Monoclonal

Antigen

Other

Description

The observed molecular weight of alpha-hCG and beta-hCG are approximately 20-22 kD & 27-32 kD respectively.

Antibody clone number

P1G7F12

Concentration

5 mg/mL

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Direct ELISA analysis of hCG proteins was performed by coating wells of a plate with a purified hCG protein extracted from human urine at a concentration of 10 µg/mL overnight at 4C. The plate was washed 3 times with ELISA Wash Buffer (Product # N503) and blocked with 300 µL of StartingBlock (PBS) Blocking Buffer (Product # 37538) for at least 1 hour at room temperature. After washing 100 µL of hCG monoclonal antibody (Product # MIH9827) was added to wells in duplicate at 10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 9.5, 0.078, 0.039, 0.019 and 0 µg/mL concentrations, and the samples were incubated for 2 hours at room temperature. The plate was washed, and then incubated with 100 µL per well of an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:10,000 for 1 hour at room temperature, and washed again with ELISA Wash Buffer. The plate was developed by incubating 100 µL per well of 1-Step Ultra TMB substrate (Product # 34028) per well for 10 minutes at room temperature in the dark. The reaction was stopped with 100 µL per well of 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.