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LearnPA1-31990
antibody from Invitrogen Antibodies
Targeting: FGFR2
BEK, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- PA1-31990 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGFR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Jurkat cell lysate
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR2/CD332 in NIH-3T3 cell line lysates (35 µg/lane) using a FGFR2/CD332 polyclonal antibody (Product # PA1-31990).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FGFR2 was performed by loading 20 µg of various whole cell lysates/extracts. Proteins were transferred to a membrane and probed with a FGFR2 Polyclonal Antibody (Product # PA1-31990) at a dilution of 1:1000-1:2000. Lane 1: DU145, Lane 2: A549, Lane 3: T47D, Lane 4: HeLa, Lane 5: K562, Lane 6: Mouse brain.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-FGFR2 Polyclonal Antibody (Product # PA1-31990) and ~80-90 kDa bands corresponding to FGFR2 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of NTERA-2 cI.D1 (Lane 1), HT-29 (Lane 2), K-562 (Lane 3), HeLa (Lane 4), SH-SY5Y (Lane 5) and THP-1 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:250 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of FGFR2 in HeLa cells using FGFR2 Polyclonal Antibody (Product # PA1-31990) (Green) at a dilution of 1:25. Red : Actin filament.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FGFR2 in human brain tissue using FGFR2 Polyclonal Antibody (Product # PA1-31990) at a dilution of 1:25.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FGFR2 in human liver tissue using FGFR2 Polyclonal Antibody (Product # PA1-31990) at a dilution of 1:25.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of FGFR2/CD332 in NCI-H460 cells (right histogram) compared to a negative control cell (left histogram) using a FGFR2/CD332 polyclonal antibody (Product # PA1-31990). Secondary detection was performed using a FITC-conjugated goat-anti-rabbit secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of SR-BI was performed in HeLa cells (fixed with 2% paraformaldehyde (PFA) (10 min), permeabilized with 90% methanol (10 min)) using a SR-BI Polyclonal Antibody (Product # PA1-31405) (Green) at a dilution of 1:25. Blue : Isotype control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of p73 was performed in HeLa cells (fixed with 2% paraformaldehyde (PFA) (10 min), permeabilized with 90% methanol (10 min)) using a p73 Monoclonal Antibody (5B429) (Product # MA1-23147) (Green) at a dilution of 1:25. Blue : Isotype control.
