GTX27256

antibody from GeneTex

Targeting: NEFM NEF3, NF-M, NFM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

GTX27256

Provider

GeneTex

Proper citation

GeneTex Cat#GTX27256, RRID:AB_366910

Product name

NF-M antibody [3H11]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western Blot: 160kDa Neurofilament Medium Antibody (3H11) (GTX27256) - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stainted with various antibodies including (GTX27256) (lane 3).

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: 160kDa Neurofilament Medium Antibody (3H11) (GTX27256) - Culture of adult neural cells grown as described (2). Mature neurons can be identified by their morphology and because they stain strongly with antibodies to NF-L, NF-M and NF-H. The surrounding stellate red cells are stained with rabbit polyclonal Rat facial nucleus before RPCA-a-Int. These are apparently mitotic neuronal progenitor cells and express many other neuronal markers