Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-35129 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHOP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references SDF4 Is a Prognostic Factor for 28-Days Mortality in Patients With Sepsis via Negatively Regulating ER Stress.
Zhu T, Su Q, Wang C, Shen L, Chen H, Feng S, Peng X, Chen S, Wang Y, Jiang H, Chen J
Frontiers in immunology 2021;12:659193
Frontiers in immunology 2021;12:659193
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GADD153 in Jurkat cell lysate (35 µg/lane) using a GADD153 polyclonal antibody (Product # PA5-35129).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of GADD153 in Jurkat cells (right) compared to a negative control (left) using a GADD153 polyclonal antibody (Product # PA5-35129) followed by detection using a FITC-conjugated donkey-anti-rabbit secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 (A) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs (p < 0.05). (B) Representative TEM picture in survival and death. (C) Representative photomicrographs showing SDF4 (green), DAPI (blue) and their merged images (original magnification x400). Scale bar, 100um. (D) GRP78 expression in survival (n=68) compared with death (n=21) in cohort. (E) Pearson correlation analysis between the expression level of GRP78 and SDF4 in cohort. (F) Pearson correlation analysis between the expression level of ATF6 and SDF4 in cohort. (G) Representative histograms of CHOP expression among survival and death in flow cytometry. (H) Mean fluorescence intensity of CHOP in survival (n=54) and death (n=19). (I) Representative histograms of ATF6 expression among survival and death in flow cytometry. (J) Mean fluorescence intensity of ATF6 in survival and death. (K) Representative histograms of GRP78 expression among survival and death in flow cytometry. (L) Mean fluorescence intensity of GRP78 in survival and death. (M) Representative photomicrographs showing CHOP (red), DAPI (blue) and their merged images (original magnification x400). Scale bar, 100um. (N) Representative photomicrographs showing ATF6 (green), DAPI (blue) and their merged images (original magnification x400). (O) Representative photomicrographs showing GRP78 (red), DAPI (blue) and their merged images (original magnification x400). ***p < 0.001, **p < 0.01, *p < 0.05.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 (A) Representative photomicrographs of Grp78 and Chop from sham-operated and CLP mice lung treated with AdCon or AdSdf4 (original magnification x400). Scale bar, 100um. (B, C) Positive staining area of Grp78 and Chop from four groups. (D) Representative western blot results from four groups for levels of cleaved- Atf6 , Grp78 , Chop , and beta-actin. (E-G) The cleaved- Atf6 , Grp78 and Chop bands were quantified by densitometry and normalized to the density of beta-actin. n=5. Data were shown in Mean +- SD. ****p < 0.001, ***p < 0.001, **p < 0.01, *p < 0.05.