12-9919-41

antibody from Invitrogen Antibodies
Targeting: FASLG APT1LG1, CD178, FasL, TNFSF6
Provider product page for 12-9919-41

Antibody data

Product number
12-9919-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), PE, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended. Applications Reported: NOK-1 has been reported for use in flow cytometric analysis. Applications Tested: This NOK-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Yellow dye
Isotype
IgG
Antibody clone number
NOK-1
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
FASLG protein structure - 12-9919-41 shown in red.