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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-804-010B-T100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-010B-T100, RRID:AB_2051398
- Product name
- FasL (mouse) monoclonal antibody (H11) (biotin conjugate)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- H11
- Vial size
- 100 tests
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Transgenic expression of CD95 ligand on thyroid follicular cells confers immune privilege upon thyroid allografts.
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL.
Characterization of the non-functional Fas ligand of gld mice.
Tourneur L, Malassagne B, Batteux F, Fabre M, Mistou S, Lallemand E, Lores P, Chiocchia G
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1338-46
Journal of immunology (Baltimore, Md. : 1950) 2001 Aug 1;167(3):1338-46
CD4+ T cells reactivated with superantigen are both more sensitive to FasL-mediated killing and express a higher level of FasL.
Wang JK, Zhu B, Ju ST, Tschopp J, Marshak-Rothstein A
Cellular immunology 1997 Aug 1;179(2):153-64
Cellular immunology 1997 Aug 1;179(2):153-64
Characterization of the non-functional Fas ligand of gld mice.
Hahne M, Peitsch MC, Irmler M, Schröter M, Lowin B, Rousseau M, Bron C, Renno T, French L, Tschopp J
International immunology 1995 Sep;7(9):1381-6
International immunology 1995 Sep;7(9):1381-6
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Detection of FasL in 293T cells transfected with a FasL expression vector. Method: Transfected cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, were incubated for 1 hr at room temperature with 20 µg/ml of biotinylated H11 antibody in PBS, 0.1% BSA. After rinsing in PBS, FITC-conjugated streptavidin was added for 30 min, slides washed again in PBS and visualized using a fluorescence microscope.
