MAB170

antibody from Novus Biologicals

Targeting: CXCR4 CD184, D2S201E, fusin, HM89, HSY3RR, LESTR, NPY3R, NPYR, NPYY3R

Provider product page for MAB170

Flow cytometry

Blocking/Neutralizing

Antibody data

Antibody Data
Antigen structure
References [34]
Comments [0]
Validations
Flow cytometry [1]
Blocking/Neutralizing [1]

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Product number: MAB170 - Provider product page
Provider: Novus Biologicals
Product name: Mouse Monoclonal CXCR4 Antibody
Antibody type: Monoclonal
Description: Protein A or G purified from ascites. Detects human CXCR4.
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Vial size: 500 ug
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

CXCR4 protein structure - MAB170 shown in red.

Submitted references Activation of MAPK Signaling by CXCR7 Leads to Enzalutamide Resistance in Prostate Cancer.

Li S, Fong KW, Gritsina G, Zhang A, Zhao JC, Kim J, Sharp A, Yuan W, Aversa C, Yang XJ, Nelson PS, Feng FY, Chinnaiyan AM, de Bono JS, Morrissey C, Rettig MB, Yu J
Cancer research 2019 May 15;79(10):2580-2592

CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells.

Ieranò C, D'Alterio C, Giarra S, Napolitano M, Rea G, Portella L, Santagata A, Trotta AM, Barbieri A, Campani V, Luciano A, Arra C, Anniciello AM, Botti G, Mayol L, De Rosa G, Pacelli R, Scala S
Cell death & disease 2019 Jul 22;10(8):562

GRK2 mediates TCR-induced transactivation of CXCR4 and TCR-CXCR4 complex formation that drives PI3Kγ/PREX1 signaling and T cell cytokine secretion.

Dinkel BA, Kremer KN, Rollins MR, Medlyn MJ, Hedin KE
The Journal of biological chemistry 2018 Sep 7;293(36):14022-14039

Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4.

Mills SC, Howell L, Beekman A, Stokes L, Mueller A
Cellular signalling 2018 Jan;42:88-96

CXCR7 maintains osteosarcoma invasion after CXCR4 suppression in bone marrow microenvironment.

Han Y, Wu C, Wang J, Liu N
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2017 May;39(5):1010428317701631

Δ20 IFITM2 differentially restricts X4 and R5 HIV-1.

Wu WL, Grotefend CR, Tsai MT, Wang YL, Radic V, Eoh H, Huang IC
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 3;114(27):7112-7117

i-bodies, Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4.

Griffiths K, Dolezal O, Cao B, Nilsson SK, See HB, Pfleger KDG, Roche M, Gorry PR, Pow A, Viduka K, Lim K, Lu BGC, Chang DHC, Murray-Rust T, Kvansakul M, Perugini MA, Dogovski C, Doerflinger M, Zhang Y, Parisi K, Casey JL, Nuttall SD, Foley M
The Journal of biological chemistry 2016 Jun 10;291(24):12641-12657

Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices.

Wescott MP, Kufareva I, Paes C, Goodman JR, Thaker Y, Puffer BA, Berdougo E, Rucker JB, Handel TM, Doranz BJ
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 30;113(35):9928-33

Heregulin/ErbB3 Signaling Enhances CXCR4-Driven Rac1 Activation and Breast Cancer Cell Motility via Hypoxia-Inducible Factor 1α.

Lopez-Haber C, Barrio-Real L, Casado-Medrano V, Kazanietz MG
Molecular and cellular biology 2016 Aug 1;36(15):2011-26

Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.

Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
PloS one 2015;10(3):e0120069

Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia.

Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari CT
Cancer research 2015 Oct 1;75(19):4153-63

The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.

Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann IS, Levine BL, June CH, Zhang L, Wang E, Marincola FM, Bedognetti D, Powell DJ Jr, Tanyi J, Feldman MD, Kandalaft LE, Coukos G
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Jun 15;21(12):2840-50

Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.

Lin YL, Yet SF, Hsu YT, Wang GJ, Hung SC
Stem cells translational medicine 2015 Jan;4(1):44-55

An isoform of C/EBPβ, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration.

Park BH, Kook S, Lee S, Jeong JH, Brufsky A, Lee BC
The Journal of biological chemistry 2013 Oct 4;288(40):28656-67

The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.

Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Jun 1;17(11):3619-30

Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells.

Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J
Experimental hematology 2008 Sep;36(9):1121-31

Endothelial NOS is required for SDF-1alpha/CXCR4-mediated peripheral endothelial adhesion of c-kit+ bone marrow stem cells.

Kaminski A, Ma N, Donndorf P, Lindenblatt N, Feldmeier G, Ong LL, Furlani D, Skrabal CA, Liebold A, Vollmar B, Steinhoff G
Laboratory investigation; a journal of technical methods and pathology 2008 Jan;88(1):58-69

Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.

Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
Journal of immunology (Baltimore, Md. : 1950) 2008 Dec 1;181(11):8053-67

Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.

Cross-talk between paracrine-acting cytokine and chemokine pathways promotes malignancy in benign human prostatic epithelium.

Ao M, Franco OE, Park D, Raman D, Williams K, Hayward SW
Cancer research 2007 May 1;67(9):4244-53

Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.

McLean AB, D'Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
Stem cells (Dayton, Ohio) 2007 Jan;25(1):29-38

CCR5-, DC-SIGN-dependent endocytosis and delayed reverse transcription after human immunodeficiency virus type 1 infection in human astrocytes.

Deiva K, Khiati A, Hery C, Salim H, Leclerc P, Horellou P, Tardieu M
AIDS research and human retroviruses 2006 Nov;22(11):1152-61

Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54.

Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A
Journal of cellular physiology 2006 May;207(2):364-73

Skin homing of Sézary cells involves SDF-1-CXCR4 signaling and down-regulation of CD26/dipeptidylpeptidase IV.

Narducci MG, Scala E, Bresin A, Caprini E, Picchio MC, Remotti D, Ragone G, Nasorri F, Frontani M, Arcelli D, Volinia S, Lombardo GA, Baliva G, Napolitano M, Russo G
Blood 2006 Feb 1;107(3):1108-15

Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells.

Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F
Stem cells (Dayton, Ohio) 2004;22(6):1015-29

Human immunodeficiency virus type 1 infection of human brain-derived progenitor cells.

Lawrence DM, Durham LC, Schwartz L, Seth P, Maric D, Major EO
Journal of virology 2004 Jul;78(14):7319-28

CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice.

Tavor S, Petit I, Porozov S, Avigdor A, Dar A, Leider-Trejo L, Shemtov N, Deutsch V, Naparstek E, Nagler A, Lapidot T
Cancer research 2004 Apr 15;64(8):2817-24

CXCR4 regulates migration and development of human acute myelogenous leukemia stem cells in transplanted NOD/SCID mice.

Tavor S, Petit I, Porozov S, Avigdor A, Dar A, Leider-Trejo L, Shemtov N, Deutsch V, Naparstek E, Nagler A, Lapidot T
Cancer research 2004 Apr 15;64(8):2817-24

Rho family small GTPases control migration of hematopoietic progenitor cells into multicellular spheroids of bone marrow stroma cells.

Bug G, Rossmanith T, Henschler R, Kunz-Schughart LA, Schröder B, Kampfmann M, Kreutz M, Hoelzer D, Ottmann OG
Journal of leukocyte biology 2002 Oct;72(4):837-45

CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.

Honczarenko M, Le Y, Glodek AM, Majka M, Campbell JJ, Ratajczak MZ, Silberstein LE
Blood 2002 Oct 1;100(7):2321-9

Is high expression of the chemokine receptor CXCR-4 of predictive value for early relapse in childhood acute lymphoblastic leukaemia?

Schneider P, Vasse M, Al Bayati A, Lenormand B, Vannier JP
British journal of haematology 2002 Nov;119(2):579-80

Is high expression of the chemokine receptor CXCR-4 of predictive value for early relapse in childhood acute lymphoblastic leukaemia?

Schneider P, Vasse M, Al Bayati A, Lenormand B, Vannier JP
British journal of haematology 2002 Nov;119(2):579-80

Expression of functional CCR and CXCR chemokine receptors in podocytes.

Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstädt H
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52

Expression of functional CCR and CXCR chemokine receptors in podocytes.

Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstädt H
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutrali-zation by Human CXCR4 Antibody. Recombinant Human/ Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND₅₀ is typically 0.3-1.2 µg/mL.