GTX48813

antibody from GeneTex

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

GTX48813

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48813, RRID:AB_11169950

Product name

MCP1 / CCL2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Bovine, Canine, Feline, Porcine

Host

Rabbit

References

Submitted references

Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.

Zera K, Zastre J
PloS one 2017;12(10):e0186707

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Chemoprevention of intestinal tumorigenesis by the natural dietary flavonoid myricetin in APCMin/+ mice.

Li Y, Cui SX, Sun SY, Shi WN, Song ZY, Wang SQ, Yu XF, Gao ZH, Qu XJ
Oncotarget 2016 Sep 13;7(37):60446-60460

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex's protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kDa (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with GeneTex's, Inc. Anti-bovine CCL2. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex's protein-A purified anti-bovine CCL2 antibody shows detection of recombinant bovine CCL2 at 8.8kDa raised in yeast. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with GeneTex's anti-bovine CCL2 antibody . After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.