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antibody from Novus Biologicals
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [48]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-479-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CCL2/JE/MCP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross-reactivity with recombinant human (rh) MCP-1, rhMCP-2, rhMCP-3, recombinant mouse (rm) MARC, rhMCP-4, rmMCP-5, rmMDC, rmMIP-1 alpha , rmMIP-1 beta , rmMIP-1 gamma , rmMIP-2, rmMIP-3 beta , rmRANTES, rmTARC, and rmTeck is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Tyrphostin AG490 reduces inflammation and fibrosis in neonatal obstructive nephropathy.
Fbxw7 increases CCL2/7 in CX3CR1hi macrophages to promote intestinal inflammation.
Selective Deletion of Heparan Sulfotransferase Enzyme, Ndst1, in Donor Endothelial and Myeloid Precursor Cells Significantly Decreases Acute Allograft Rejection.
Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis.
Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration.
The Chemokine CCL2 Mediates the Seizure-enhancing Effects of Systemic Inflammation.
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice.
P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation.
IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Persistent inflammation subverts thrombospondin-1-induced regulation of retinal angiogenesis and is driven by CCR2 ligation.
Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Megalin contributes to the early injury of proximal tubule cells during nonselective proteinuria.
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Thrombin induces fibroblast CCL2/JE production and release via coupling of PAR1 to Galphaq and cooperation between ERK1/2 and Rho kinase signaling pathways.
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis.
Chemical interactions between fibrosarcoma cancer cells and sensory neurons contribute to cancer pain.
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
Sequential ELISA to profile multiple cytokines from small volumes.
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Selective macrophage suppression during sepsis.
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
A critical role for CCR2/MCP-1 interactions in the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation.
Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas.
Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas.
The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Gasparitsch M, Schieber A, Schaubeck T, Keller U, Cattaruzza M, Lange-Sperandio B
PloS one 2019;14(12):e0226675
PloS one 2019;14(12):e0226675
Fbxw7 increases CCL2/7 in CX3CR1hi macrophages to promote intestinal inflammation.
He J, Song Y, Li G, Xiao P, Liu Y, Xue Y, Cao Q, Tu X, Pan T, Jiang Z, Cao X, Lai L, Wang Q
The Journal of clinical investigation 2019 Jun 27;129(9):3877-3893
The Journal of clinical investigation 2019 Jun 27;129(9):3877-3893
Selective Deletion of Heparan Sulfotransferase Enzyme, Ndst1, in Donor Endothelial and Myeloid Precursor Cells Significantly Decreases Acute Allograft Rejection.
Chen H, Ambadapadi S, Wakefield D, Bartee M, Yaron JR, Zhang L, Archer-Hartmann SA, Azadi P, Burgin M, Borges C, Zheng D, Ergle K, Muppala V, Morshed S, Rand K, Clapp W, Proudfoot A, Lucas A
Scientific reports 2018 Sep 7;8(1):13433
Scientific reports 2018 Sep 7;8(1):13433
Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis.
Zou A, Magee N, Deng F, Lehn S, Zhong C, Zhang Y
The Journal of biological chemistry 2018 Jun 1;293(22):8656-8671
The Journal of biological chemistry 2018 Jun 1;293(22):8656-8671
Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration.
Karlen SJ, Miller EB, Wang X, Levine ES, Zawadzki RJ, Burns ME
Journal of neuroinflammation 2018 Dec 15;15(1):344
Journal of neuroinflammation 2018 Dec 15;15(1):344
The Chemokine CCL2 Mediates the Seizure-enhancing Effects of Systemic Inflammation.
Cerri C, Genovesi S, Allegra M, Pistillo F, Püntener U, Guglielmotti A, Perry VH, Bozzi Y, Caleo M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Mar 30;36(13):3777-88
The Journal of neuroscience : the official journal of the Society for Neuroscience 2016 Mar 30;36(13):3777-88
Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Denney L, Byrne AJ, Shea TJ, Buckley JS, Pease JE, Herledan GM, Walker SA, Gregory LG, Lloyd CM
Immunity 2015 Nov 17;43(5):945-58
Immunity 2015 Nov 17;43(5):945-58
Vitamin D limits chemokine expression in adipocytes and macrophage migration in vitro and in male mice.
Karkeni E, Marcotorchino J, Tourniaire F, Astier J, Peiretti F, Darmon P, Landrier JF
Endocrinology 2015 May;156(5):1782-93
Endocrinology 2015 May;156(5):1782-93
P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
Wang H, Hong LJ, Huang JY, Jiang Q, Tao RR, Tan C, Lu NN, Wang CK, Ahmed MM, Lu YM, Liu ZR, Shi WX, Lai EY, Wilcox CS, Han F
Cell research 2015 Jun;25(6):674-90
Cell research 2015 Jun;25(6):674-90
GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation.
Zhao C, Bu X, Wang W, Ma T, Ma H
PloS one 2014;9(1):e85058
PloS one 2014;9(1):e85058
IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells.
Li S, Wang W, Zhang N, Ma T, Zhao C
BMC cancer 2014 Jul 3;14:480
BMC cancer 2014 Jul 3;14:480
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Persistent inflammation subverts thrombospondin-1-induced regulation of retinal angiogenesis and is driven by CCR2 ligation.
Chen M, Copland DA, Zhao J, Liu J, Forrester JV, Dick AD, Xu H
The American journal of pathology 2012 Jan;180(1):235-45
The American journal of pathology 2012 Jan;180(1):235-45
Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
PloS one 2011;6(7):e22504
PloS one 2011;6(7):e22504
Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
Kim D, You B, Lim H, Lee SJ
Molecular pain 2011 Sep 28;7:74
Molecular pain 2011 Sep 28;7:74
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
International journal of cancer 2009 Sep 15;125(6):1276-84
International journal of cancer 2009 Sep 15;125(6):1276-84
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology 2008 Oct;125(2):272-9
Immunology 2008 Oct;125(2):272-9
Megalin contributes to the early injury of proximal tubule cells during nonselective proteinuria.
Motoyoshi Y, Matsusaka T, Saito A, Pastan I, Willnow TE, Mizutani S, Ichikawa I
Kidney international 2008 Nov;74(10):1262-9
Kidney international 2008 Nov;74(10):1262-9
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3277-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3277-90
Thrombin induces fibroblast CCL2/JE production and release via coupling of PAR1 to Galphaq and cooperation between ERK1/2 and Rho kinase signaling pathways.
Deng X, Mercer PF, Scotton CJ, Gilchrist A, Chambers RC
Molecular biology of the cell 2008 Jun;19(6):2520-33
Molecular biology of the cell 2008 Jun;19(6):2520-33
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
The Journal of clinical investigation 2008 Jan;118(1):183-94
The Journal of clinical investigation 2008 Jan;118(1):183-94
Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis.
Basso AS, Frenkel D, Quintana FJ, Costa-Pinto FA, Petrovic-Stojkovic S, Puckett L, Monsonego A, Bar-Shir A, Engel Y, Gozin M, Weiner HL
The Journal of clinical investigation 2008 Apr;118(4):1532-43
The Journal of clinical investigation 2008 Apr;118(4):1532-43
Chemical interactions between fibrosarcoma cancer cells and sensory neurons contribute to cancer pain.
Khasabova IA, Stucky CL, Harding-Rose C, Eikmeier L, Beitz AJ, Coicou LG, Hanson AE, Simone DA, Seybold VS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Sep 19;27(38):10289-98
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Sep 19;27(38):10289-98
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
Journal of immunology (Baltimore, Md. : 1950) 2007 Dec 1;179(11):7949-58
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
The Journal of experimental medicine 2006 Nov 27;203(12):2763-77
The Journal of experimental medicine 2006 Nov 27;203(12):2763-77
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7296-302
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):612-20
CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 15;177(4):2651-61
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 15;177(4):2651-61
CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 15;177(4):2651-61
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 15;177(4):2651-61
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Osuchowski MF, Welch K, Siddiqui J, Remick DG
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Journal of immunology (Baltimore, Md. : 1950) 2006 Aug 1;177(3):1967-74
Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
Mbebi C, González de Aguilar JL, Sée V, Dupuis L, Frossard N, Mercken L, Pradier L, Larmet Y, Loeffler JP
Neurobiology of disease 2005 Jun-Jul;19(1-2):129-41
Neurobiology of disease 2005 Jun-Jul;19(1-2):129-41
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Perrin FE, Lacroix S, Avilés-Trigueros M, David S
Brain : a journal of neurology 2005 Apr;128(Pt 4):854-66
Brain : a journal of neurology 2005 Apr;128(Pt 4):854-66
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood 2004 Sep 1;104(5):1404-10
Blood 2004 Sep 1;104(5):1404-10
A critical role for CCR2/MCP-1 interactions in the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation.
Hildebrandt GC, Duffner UA, Olkiewicz KM, Corrion LA, Willmarth NE, Williams DL, Clouthier SG, Hogaboam CM, Reddy PR, Moore BB, Kuziel WA, Liu C, Yanik G, Cooke KR
Blood 2004 Mar 15;103(6):2417-26
Blood 2004 Mar 15;103(6):2417-26
Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas.
Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A
Immunology letters 2002 Jun 3;82(1-2):111-21
Immunology letters 2002 Jun 3;82(1-2):111-21
Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas.
Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A
Immunology letters 2002 Jun 3;82(1-2):111-21
Immunology letters 2002 Jun 3;82(1-2):111-21
The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
The Journal of clinical investigation 2001 Nov;108(9):1307-14
The Journal of clinical investigation 2001 Nov;108(9):1307-14
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Mouse CCL2/JE/MCP-1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (Catalog # 456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/ MCP-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND50 is typically 0.5-2.5 µg/mL.
