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antibody from R&D Systems
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- BAF1774 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine CCL2/JE/MCP-1 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine CCL2/JE/MCP-1 in ELISAs and Western blots. In sandwich immunoassay, less than 0.05% cross-reactivity with recombinant mouse MCP-1 and recombinant human MCP-1 is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P52203- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL2/JE/MCP-1 in Canine PBMCs. CCL2/JE/MCP-1 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine CCL2/JE/MCP-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1774) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
