- 16-7096-85 - Invitrogen Antibodies CCL2 antibody

Submitted references Mas receptor activation attenuates allergic airway inflammation via inhibiting JNK/CCL2-induced macrophage recruitment.

Hong L, Wang Q, Chen M, Shi J, Guo Y, Liu S, Pan R, Yuan X, Jiang S
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 May;137:111365

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Zheng C, Sui B, Zhang X, Hu J, Chen J, Liu J, Wu D, Ye Q, Xiang L, Qiu X, Liu S, Deng Z, Zhou J, Liu S, Shi S, Jin Y
Journal of extracellular vesicles 2021 May;10(7):e12109

Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

Berry MR, Mathews RJ, Ferdinand JR, Jing C, Loudon KW, Wlodek E, Dennison TW, Kuper C, Neuhofer W, Clatworthy MR
Cell 2017 Aug 24;170(5):860-874.e19

Brain Invasion by Mouse Hepatitis Virus Depends on Impairment of Tight Junctions and Beta Interferon Production in Brain Microvascular Endothelial Cells.

Bleau C, Filliol A, Samson M, Lamontagne L
Journal of virology 2015 Oct;89(19):9896-908

Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis.

Shen C, Lie P, Miao T, Yu M, Lu Q, Feng T, Li J, Zu T, Liu X, Li H
Molecular medicine reports 2015 Jul;12(1):20-30

The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells.

Nijnik A, Pistolic J, Cho P, Filewod NC, Falsafi R, Ramin A, Harder KW, Hancock RE
Journal of leukocyte biology 2012 Apr;91(4):599-607

Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.

Nijnik A, Madera L, Ma S, Waldbrook M, Elliott MR, Easton DM, Mayer ML, Mullaly SC, Kindrachuk J, Jenssen H, Hancock RE
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2539-50

Mechanisms underlying renoprotection during renin-angiotensin system blockade.

Taal MW, Chertow GM, Rennke HG, Gurnani A, Jiang T, Shahsafaei A, Troy JL, Brenner BM, Mackenzie HS
American journal of physiology. Renal physiology 2001 Feb;280(2):F343-55

Mechanisms underlying renoprotection during renin-angiotensin system blockade.

Taal MW, Chertow GM, Rennke HG, Gurnani A, Jiang T, Shahsafaei A, Troy JL, Brenner BM, Mackenzie HS
American journal of physiology. Renal physiology 2001 Feb;280(2):F343-55

Macrophage inflammatory protein-2 and KC induce chemokine production by mouse astrocytes.

Luo Y, Fischer FR, Hancock WW, Dorf ME
Journal of immunology (Baltimore, Md. : 1950) 2000 Oct 1;165(7):4015-23

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 3 Efferocytosis of MSC-derived apoVs by liver macrophages alleviates macrophage infiltration in the type 2 diabetes (T2D) liver. (a) Representative confocal microscopy images showing distribution of PKH26-labeled apoVs (red) in the liver, counterstained by Hoechst (blue). After removal of unbound PKH, the stained apoVs were resuspended in PBS and underwent centrifugation, after which the supernatant was used as the negative control (NC) and injected. Scale bars, 50 mum. (b) Representative confocal microscopy images showing uptake of apoVs (red) by macrophages (green) in the liver, counterstained by Hoechst (blue). Scale bars, 50 mum in low magnification images and 25 mum in high magnification images. (c) Flow cytometric analysis showing the uptake of apoVs by macrophages in the liver. KCs, Kupffer cells; MoMFs, monocyte-derived macrophages. (d) Representative immunofluorescent (IF) staining images of F4/80 (green) and CD11b (green) in the liver, counterstained by Hoechst (blue). Ctrl, control mice; DIO, mice with diet-induced obesity; apoV, DIO mice treated by apoVs. Scale bars, 50 mum. (e) Flow cytometric analysis and the corresponding quantification of the percentages of KCs and MoMFs in hepatic CD45 + cells. N = 6 per group. (f) Representative IF staining images of chemokine (C-C motif) ligand 2 (CCL2) (red) in the liver, counterstained by Hoechst (blue). Scale bars, 50 mum. (g) Enzyme-linked immunosorbent assay (ELISA) analysis of CCL2 in liver lysate and serum. N

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 8 CRT mediates efferocytosis of MSC-derived apoVs to modulate T2D liver macrophages in vivo. (a) Representative immunofluorescent (IF) staining images of F4/80 (green) and CD11b (green) in the liver, counterstained by Hoechst (blue). DIO, mice with diet-induced obesity; si-NC-apoV, DIO mice treated by apoVs derived from MSCs transfected by siRNA-negative control; si- CRT -apoV, DIO mice treated by apoVs derived from MSCs transfected by siRNA- CRT . Scale bars, 50 mum. (b) Flow cytometric analysis and the corresponding quantification of the percentages of KCs and MoMFs in hepatic CD45 + cells. KCs, Kupffer cells; MoMFs, monocyte-derived macrophages. N = 5-6 per group. (c) Representative IF staining images of chemokine (C-C motif) ligand 2 (CCL2) (red) in the liver, counterstained by Hoechst (blue). Scale bars, 50 mum. (d and e) ELISA analysis of CCL2 in liver lysate (d) and serum (e). N = 6 per group. (f and g) Representative IF staining images of tumor necrosis factor-alpha (TNF-alpha) (red) and CD206 (green) in the liver, counterstained by Hoechst (blue), and the corresponding quantification of fold changes over the DIO group. Scale bars, 50 mum. N = 5-6 per group. (h and i) Quantitative real time polymerase chain reaction (qRT-PCR) analysis of mRNA expression levels of Tnf (h) and interleukin 10 ( Il10 ) (i) in the liver, normalized to beta-actin ( Actb ), and quantification of fold changes over the DIO group. N = 5 per group. (j and k) Enzyme-linked immunosorbent as

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Migration of fibroblasts, HUVECs and UC-MSCs in response to UC-CM. (A) A total of 5x10 4 cells were collected and allowed to migrate. Lane 1, UC-CM; lane 2, DMEM; lanes 3-6, in the presence or absence of anti-SDF-1 (20 mu g/ml), anti-MCP-1 (20 mu g/ml) or anti-HGF (20 mu g/ml), respectively. Results are from a representative experiment and are expressed as the mean number of migrated cells in three random fields, scale bar=200 mu m. Cells that crossed the matrigel membrane were stained with crystal violet (magnification, x40). (B) Graphical presentation of the quantified data, presented as the number of migrated cells and expressed as the mean +- standard error of the mean. HUVECs, human umbilical vein endothelial cells; UC-MSCs, umbilical cord mesenchymal stem cells; UC-CM, UC-MSCs conditioned medium; DMEM, Dulbecco's modified Eagle's medium; SDF-1, stromal cell-derived factor 1; MCP-1, monocyte chemotactic protein 1; HGF, hepatocyte growth factor.

16-7096-85

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