PA5-21417

antibody from Invitrogen Antibodies

Targeting: PHB2 Bap37, BCAP37, p22, REA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21417

Provider

Invitrogen Antibodies

Product name

REA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: H1299, HeLa, mouse brain.

Concentration

0.66 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using REA Polyclonal Antibody (Product # PA5-21417). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 12% SDS PAGE. REA Polyclonal Antibody (Product # PA5-21417) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using REA Polyclonal Antibody (Product # PA5-21417). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: HeLa. 10% SDS PAGE. REA Polyclonal Antibody (Product # PA5-21417) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of REA in paraformaldehyde-fixed A431 cells using a REA polyclonal antibody (Product # PA5-21417) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA antibody (Product # PA5-21417) at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using REA antibody (Product # PA5-21417) at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

REA Polyclonal Antibody detects PHB2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. REA Polyclonal Antibody (Product # PA5-21417) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

REA Polyclonal Antibody detects Prohibitin 2 protein at cytoplasm and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. REA Polyclonal Antibody (Product # PA5-21417) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.