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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- MA5-24985 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LTA4H Monoclonal Antibody (OTI8F4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI8F4
- Vial size
- 100 µL
- Concentration
- 1.3 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed on HEK293T cells overexpressing LTA4H. Antigen-antibody complexes were formed by incubating the sample with 2 µg of LTA4H monoclonal antibody (Product # MA5-24985) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. The sample was washed extensively and probed with rabbit anti-DDK polyclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LTA4H in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with LTA4H (Product # MA5-24985) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LTA4H in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with LTA4H (Product # MA5-24985) monoclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LTA4H in human lung tissue. Tissue were probed with a IdU monoclonal antibody (Product # MA5-24985).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of LTA4H in human lung tissue. Tissue were probed with a IdU monoclonal antibody (Product # MA5-24985).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a LTA4H monoclonal antibody (Product # MA5-24985) at a dilution of 1:50.
