22480002-0.1mg

antibody from Novus Biologicals

Targeting: DEK D6S231E

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

22480002-0.1mg

Provider

Novus Biologicals

Product name

Rabbit Polyclonal DEK Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This product is specific for Human DEK.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DEK Antibody [22480002] - Western Blot was performed using affinity purified SEQer DEK antibody, aa(226-325) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer DEK antibody, aa(226-325) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DEK Antibody [22480002] - Samples: Lane 1, Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil Target weight [kDa]: 48, 43 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: DEK Antibody [22480002]