H00004605-D01P

antibody from Abnova Corporation

Targeting: MYBL2 B-MYB, BMYB

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004605-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004605-D01P, RRID:AB_1577528

Product name

MYBL2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MYBL2 protein.

Antigen sequence

MSRRTRCEDLDELHYQDTDSDVPEQRDSKCKVKWT
HEEDEQLRALVRQFGQQDWKFLASHFPNRTDQQCQ
YRWLRVLNPDLVKGPWTKEEDQKVIELVKKYGTKQ
WTLIAKHLKGRLGKQCRERWHNHLNPEVKKSCWTE
EEDRIICEAHKVLGNRWAEIAKMLPGRTDNAVKNH
WNSTIKRKVDTGGFLSESKDCKPPVYLLLELEDKD
GLQSAQPTEGQGSLLTNWPSVPPTIKEEENSEEEL
AAATTSKEQEPIGTDLDAVRTPEPLEEFPKREDQE
GSPPETSLPYKWVVEAANLLIPAVGSSLSEALDLI
ESDPDAWCDLSKFDLPEEPSAEDSINNSLVQLQAS
HQQQVLPPRQPSALVPSVTEYRLDGHTISDLSRSS
RGELIPISPSTEVGGSGIGTPPSVLKRQRKRRVAL
SPVTENSTSLSFLDSCNSLTPKSTPVKTLPFSPSQ
FLNFWNKQDTLELESPSLTSTPVCSQKVVVTTPLH
RDKTPLHQKHAAFVTPDQKYSMDNTPHTPTPFKNA
LEKYGPLKPLPQTPHLEEDLKEVLRSEAGIELIIE
DDIRPEKQKRKPGLRRSPIKKVRKSLALDIVDEDV
KLMMSTLPKSLSLPTTAPSNSSSLTLSGIKEDNSL
LNQGFLQAKPEKAAVAQKPRSHFTTPAPMSSAWKT
VACGGTRDQLFMQEKARQLLGRLKPSHTSRTLILS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MYBL2 expression in transfected 293T cell line (H00004605-T01) by MYBL2 MaxPab polyclonal antibody.Lane 1: MYBL2 transfected lysate(78.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MYBL2 and E2F1. Mahlavu cells were stained with anti-MYBL2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MYBL2 and E2F1. HeLa cells were stained with anti-MYBL2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)