AF3360

antibody from Novus Biologicals

Targeting: TAL1 bHLHa17, SCL, TCL5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF3360

Provider

Novus Biologicals

Product name

Goat Polyclonal SCL/Tal1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human SCL in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Inhibition of all-trans retinoic acid-induced granulocytic differentiation of WEHI-3B D+ cells by forced expression of SCL (TAL1) and GATA-1.

Ishiguro K, Rice AM, Rice KP, Sartorelli AC
Leukemia research 2009 Sep;33(9):1249-54

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human SCL/Tal1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.