PA5-46840

antibody from Invitrogen Antibodies

Targeting: TAL1 bHLHa17, SCL, TCL5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46840

Provider

Invitrogen Antibodies

Product name

TAL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: QDVLSPNSSC GSSLDGAASP DSYTEEPAPK HTARSLHPAM LPAADGAGPR

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human fetal muscle cell lysate using an anti-TAL-1 polyclonal antibody (Product # PA5-46840).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-TAL1 Polyclonal Antibody (Product # PA5-46840) and 40 kDa band corresponding to TAL1 was observed across the cell lines tested except HL-60, HCT 116 and OVCAR-3 which are reported to be negative. An uncharacterized band of ~ 55 kDa was observed in negative cell lines. Modified whole cell extracts (1% SDS) (30 µg lysate) of HEL 92.1.7 (Lane 1), K-562 (Lane 2), Jurkat (Lane 3), MOLT-4 (Lane 4), HL-60 (Lane 5), HCT 116 (Lane 6), OVCAR-3 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (paraffin-embedded) analysis of human breast cells using an anti-TAL-1 polyclonal antibody (Product # PA5-46840).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (paraffin-embedded) analysis of human breast cells using an anti-TAL-1 polyclonal antibody (Product # PA5-46840).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (paraffin-embedded) analysis of human hepatocyte tissue using an anti-TAL-1 polyclonal antibody (Product # PA5-46840).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (paraffin-embedded) analysis of human liver tissue using an anti-TAL-1 polyclonal antibody (Product # PA5-46840).