Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB3214 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat EGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat EGF in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Epigen, rmEpiregulin, recombinant human (rh) HB-EGF, rhEGF, rmEGF, or rhHRG is observed.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P07522
- Isotype
- IgG
- Antibody clone number
- 420610
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by EGF and Neutralization by Rat EGF Antibody. Recombinant Rat EGF (Catalog # 3214-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat EGF (75 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat EGF Monoclonal Antibody (Catalog # MAB3214). The ND50 is typically 0.75-3.75 µg/mL.