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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF2028 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse EGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human EGF is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A critical role for NF2 and the Hippo pathway in branching morphogenesis.
VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury.
Leukotriene B4 type-1 receptor signaling promotes liver repair after hepatic ischemia/reperfusion injury through the enhancement of macrophage recruitment.
Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
Reginensi A, Enderle L, Gregorieff A, Johnson RL, Wrana JL, McNeill H
Nature communications 2016 Aug 2;7:12309
Nature communications 2016 Aug 2;7:12309
VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury.
Ohkubo H, Ito Y, Minamino T, Eshima K, Kojo K, Okizaki S, Hirata M, Shibuya M, Watanabe M, Majima M
PloS one 2014;9(8):e105533
PloS one 2014;9(8):e105533
Leukotriene B4 type-1 receptor signaling promotes liver repair after hepatic ischemia/reperfusion injury through the enhancement of macrophage recruitment.
Ohkubo H, Ito Y, Minamino T, Mishima T, Hirata M, Hosono K, Shibuya M, Yokomizo T, Shimizu T, Watanabe M, Majima M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):3132-43
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):3132-43
Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S
The Journal of clinical investigation 2011 May;121(5):1692-708
The Journal of clinical investigation 2011 May;121(5):1692-708
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EGF in Mouse Kidney. EGF was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by EGF and Neutralization by Mouse EGF Antibody. Recombinant Mouse EGF (Catalog # 2028-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse EGF (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028). The ND50 is typically 0.02-0.06 µg/mL.
