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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB464 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Sonic Hedgehog/Shh Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects the N-terminal peptide of human (amino acids 24-197) and mouse (amino acids 25-198) Shh in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with the N-terminal peptides of recombinant mouse (rm) Dhh (amino acids 23-198) and rmIhh (amino acids 66-240) and no cross-reactivity with the C-terminal peptides from rmShh (amino acids 199-437), rmDhh (amino acids 199-396), or rmIhh (amino acids 241-449).
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Pancreatic tumor cell secreted CCN1/Cyr61 promotes endothelial cell migration and aberrant neovascularization.
Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury.
Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells.
Maity G, Mehta S, Haque I, Dhar K, Sarkar S, Banerjee SK, Banerjee S
Scientific reports 2014 May 16;4:4995
Scientific reports 2014 May 16;4:4995
Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury.
Hashimoto M, Ishii K, Nakamura Y, Watabe K, Kohsaka S, Akazawa C
Journal of neurochemistry 2008 Nov;107(4):918-27
Journal of neurochemistry 2008 Nov;107(4):918-27
Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells.
Bänziger C, Soldini D, Schütt C, Zipperlen P, Hausmann G, Basler K
Cell 2006 May 5;125(3):509-22
Cell 2006 May 5;125(3):509-22
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
