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- Antigen structure
- References [6]
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- Validations
- Immunohistochemistry [2]
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- Product number
- BAF1300 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal MICA Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human MICA in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross reactivity with recombinant human (rh) MICB and rhMIC1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS.
NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
Raneros AB, Minguela A, Rodriguez RM, Colado E, Bernal T, Anguita E, Mogorron AV, Gil AC, Vidal-Castiñeira JR, Márquez-Kisinousky L, Bulnes PD, Marin AM, Garay MCG, Suarez-Alvarez B, Lopez-Larrea C
Oncotarget 2017 May 9;8(19):31959-31976
Oncotarget 2017 May 9;8(19):31959-31976
NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
Spada R, Rojas JM, Pérez-Yagüe S, Mulens V, Cannata-Ortiz P, Bragado R, Barber DF
Journal of leukocyte biology 2015 Mar;97(3):583-98
Journal of leukocyte biology 2015 Mar;97(3):583-98
Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
Esteso G, Luzón E, Sarmiento E, Gómez-Caro R, Steinle A, Murphy G, Carbone J, Valés-Gómez M, Reyburn HT
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1344-52
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1344-52
The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
Nückel H, Switala M, Sellmann L, Horn PA, Dürig J, Dührsen U, Küppers R, Grosse-Wilde H, Rebmann V
Leukemia 2010 Jun;24(6):1152-9
Leukemia 2010 Jun;24(6):1152-9
Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS.
Guerra S, González JM, Climent N, Reyburn H, López-Fernández LA, Nájera JL, Gómez CE, García F, Gatell JM, Gallart T, Esteban M
Journal of virology 2010 Aug;84(16):8141-52
Journal of virology 2010 Aug;84(16):8141-52
NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A
Blood 2008 Feb 1;111(3):1428-36
Blood 2008 Feb 1;111(3):1428-36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MICA in Human Colon. MICA was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human MICA Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1300) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- MICA in Human Colon. MICA was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human MICA Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1300) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
