AM1486b

antibody from Abgent

Targeting: NANOG FLJ12581, FLJ40451

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1486b

Provider

Abgent

Proper citation

Abgent Cat#AM1486b, RRID:AB_2150390

Product name

NANOG Antibody

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.49 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-NANOG monoclonal antibody (Cat.#AM1486b) by NONAG recombinant protein. NANOG (NANOG + His tag)(arrow) was detected using the Mab.

Primary Ab dilution

1:100~250

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

NANOG (Cat. #AM1486b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).

Primary Ab dilution

1:100~250

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

NANOG Antibody (Cat. #AM1486b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOG Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:10~50