MA1-017-D550

antibody from Invitrogen Antibodies

Targeting: NANOG FLJ12581, FLJ40451

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-017-D550

Provider

Invitrogen Antibodies

Product name

Nanog Monoclonal Antibody (23D2-3C6), DyLight™ 550

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-017-D550 has been successfully used in ICC/IF applications with human samples.

Conjugate

Yellow dye

Antibody clone number

23D2-3C6

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Nanog using DyLight 550 directly conjugated anti-Nanog monoclonal antibody. Human iPSC Ad1 cells grown on vitronectin were fixed, permeabilized and stained with DyLight 550 conjugated anti-Nanog (Product # MA1-017-D550). Nuclear DNA was stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nanog (red) in human induced pluripotent stem cells. The cells were fixed, permeabilized and stained with a DyLight 488 conjugated SSEA3 monoclonal antibody (Product # MA1-020-D488, green) and a DyLight D550 conjugated NANOG monoclonal antibody (Product # MA1-017-D550, red) at a dilution of 1:100 in 3% BSA/PBS blocking buffer overnight at 4°C. Note: Data courtesy of Innovators Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Nanog using DyLight 550 directly conjugated anti-Nanog monoclonal antibody. Human iPSC Ad1 cells grown on vitronectin were fixed, permeabilized and stained with DyLight 550 conjugated anti-Nanog (Product # MA1-017-D550). Nuclear DNA was stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nanog (red) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 550-conjugated Nanog monoclonal antibody (Product # MA1-017-D550) in 3% BSA-PBS at a dilution of 1:50 and incubated for 1 hour at 37C in the dark. Nuclei (left panel, blue) were stained with DAPI. Images were taken at 60X magnification.