NBP1-47271

antibody from Novus Biologicals

Targeting: PQBP1 MRX2, MRX55, MRXS3, MRXS8, RENS1, SHS

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-47271

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-47271, RRID:AB_10010225

Product name

Rabbit Polyclonal PQBP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: PQBP1 Antibody [NBP1-47271] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:200 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit