Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-35360 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-BMI-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BMI1 in MCF-7 cells using a BMI1 polyclonal antibody (Product # PA5-35360) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue) and cytoplasmic actin was stained with a fluorescent phalloidin (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BMI1 in formalin-fixed, paraffin-embedded human breast carcinoma using a BMI1 polyclonal antibody (Product # PA5-35360) followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of BMI1 in MCF-7 cells (bottom) compared to a negative control (top) using a BMI1 polyclonal antibody (Product # PA5-35360) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.