Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [4]
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Validation data
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- Product number
- MA5-14943 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (F.971.6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Drosophila, Yeast
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- F.971.6
- Vial size
- 100 µL
- Concentration
- 293 µg/mL
- Storage
- -20°C
Submitted references A Mesh-Duox pathway regulates homeostasis in the insect gut.
Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis.
ROS-mediated activation of JNK/p38 contributes partially to the pro-apoptotic effect of ajoene on cells of lung adenocarcinoma.
Xiao X, Yang L, Pang X, Zhang R, Zhu Y, Wang P, Gao G, Cheng G
Nature microbiology 2017 Mar 1;2:17020
Nature microbiology 2017 Mar 1;2:17020
Kimchi attenuates fatty streak formation in the aorta of low-density lipoprotein receptor knockout mice via inhibition of endoplasmic reticulum stress and apoptosis.
Woo M, Kim M, Noh JS, Park CH, Song YO
Nutrition research and practice 2017 Dec;11(6):445-451
Nutrition research and practice 2017 Dec;11(6):445-451
ROS-mediated activation of JNK/p38 contributes partially to the pro-apoptotic effect of ajoene on cells of lung adenocarcinoma.
Wang Y, Sun Z, Chen S, Jiao Y, Bai C
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Mar;37(3):3727-38
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Mar;37(3):3727-38
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SAPK/JNK (pThr183/Tyr185) was performed by loading 20 µg of THP-1 whole cell lysates, from cells either left untreated (left lane) as a control (C) or treated with 0.3M H2O2 (right lane) for 30 minutes, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-14943) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Anisomycin (25uM for 30 min) (Lane 2), HEK293T (Lane 3), HEK293T treated with Anisomycin (25uM for 30 min) (Lane 4), NIH/3T3 (Lane 5) and NIH/3T3 treated with TNF alpha (5 ng/mL for 15 min) (Lane 6). The blot was probed with Anti-Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (F.971.6) (Product # MA5-14943, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 46 and 55 kDa band corresponding to Phospho-JNK1/JNK2 (Thr183, Tyr185) was observed upon treatment in all the cell lines.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-SAPK/JNK pThr183/Tyr185 in paraffin-embedded 293T cells, untreated (left) or UV-treated (right), using a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-14943).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Phospho-SAPK/JNK (pThr183/Tyr185) was performed on HEK293T cells. Antigen-antibody complexes were formed by incubating 500 µg of HEK293T whole cell lysate (in 500 µL volume) with 5 µL of a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-14943, Lane 2) or a rabbit IgG isotype control (Lane 1) overnight at 4°C. The immune complexes were captured on 30 µL of protein G agarose, washed extensively, and eluted with 6X Laemmli buffer. Samples were resolved on a 10% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-SAPK/JNK pThr183/Tyr185 Antibody (Product # MA5-14943) at a dilution of 1:1000 for overnight at 4°C, washed in TBST, and probed with an HRP-conjugated mouse anti-rabbit light chain secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Phospho-SAPK/JNK (pThr183/Tyr185) was performed on HEK293T cells. Antigen-antibody complexes were formed by incubating 500 µg of HEK293T whole cell lysate (in 500 µL volume) with 5 µL of a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-14943, Lane 2) or a rabbit IgG isotype control (Lane 1) overnight at 4°C. The immune complexes were captured on 30 µL of protein G agarose, washed extensively, and eluted with 6X Laemmli buffer. Samples were resolved on a 10% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-SAPK/JNK pThr183/Tyr185 Antibody (Product # MA5-14943) at a dilution of 1:1000 for overnight at 4°C, washed in TBST, and probed with an HRP-conjugated mouse anti-rabbit light chain secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL