NB100-64979

antibody from Novus Biologicals

Targeting: TFRC CD71, p90, TFR1

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Functional assay (Data presented on provider website)

Antibody Data

Product number

NB100-64979

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-64979, RRID:AB_962622

Product name

Rat Monoclonal TfR (Transferrin R) Antibody

Antibody type

Monoclonal

Description

Protein G purified. NB100-64979 recognizes native, soluble and denatured forms of murine CD71. The antibody has been used as a BBB transporter vector in mice and is suitable for studying CD71 expression, and iron uptake into different tissues, in the mouse.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

0.25 mg

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell Specialization.

Yanagida K, Engelbrecht E, Niaudet C, Jung B, Gaengel K, Holton K, Swendeman S, Liu CH, Levesque MV, Kuo A, Fu Z, Smith LEH, Betsholtz C, Hla T
Developmental cell 2020 Mar 23;52(6):779-793.e7

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An Affibody Molecule Is Actively Transported into the Cerebrospinal Fluid via Binding to the Transferrin Receptor.

Meister SW, Hjelm LC, Dannemeyer M, Tegel H, Lindberg H, Ståhl S, Löfblom J
International journal of molecular sciences 2020 Apr 23;21(8)

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Erythropoietin stimulates fibroblast growth factor 23 (FGF23) in mice and men.

Daryadel A, Bettoni C, Haider T, Imenez Silva PH, Schnitzbauer U, Pastor-Arroyo EM, Wenger RH, Gassmann M, Wagner CA
Pflugers Archiv : European journal of physiology 2018 Oct;470(10):1569-1582

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Comparative binding, endocytosis, and biodistribution of antibodies and antibody-coated carriers for targeted delivery of lysosomal enzymes to ICAM-1 versus transferrin receptor.

Papademetriou J, Garnacho C, Serrano D, Bhowmick T, Schuchman EH, Muro S
Journal of inherited metabolic disease 2013 May;36(3):467-77

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In vivo performance of polymer nanocarriers dually-targeted to epitopes of the same or different receptors.

Papademetriou IT, Garnacho C, Schuchman EH, Muro S
Biomaterials 2013 Apr;34(13):3459-66

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Differential axial localization along the mouse brain vascular tree of luminal sodium-dependent glutamine transporters Snat1 and Snat3.

Ruderisch N, Virgintino D, Makrides V, Verrey F
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2011 Jul;31(7):1637-47

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Targeting rat anti-mouse transferrin receptor monoclonal antibodies through blood-brain barrier in mouse.

Lee HJ, Engelhardt B, Lesley J, Bickel U, Pardridge WM
The Journal of pharmacology and experimental therapeutics 2000 Mar;292(3):1048-52

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Immunohistochemical localization of the murine transferrin receptor (TfR) on blood-tissue barriers using a novel anti-TfR monoclonal antibody.

Kissel K, Hamm S, Schulz M, Vecchi A, Garlanda C, Engelhardt B
Histochemistry and cell biology 1998 Jul;110(1):63-72

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: TfR (Transferrin R) Antibody (8D3) [NB100-64979] - RPE conjugated rat anti mouse Ter-119 and FITC conjugated Rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse Ter-119 and FITC conjugated rat anti mouse CD71. All experiments performed on mouse bone marrow. Data acquired on the ZE5Cell Analyzer