Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [6]
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Validation data
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- Product number
- MA5-16643 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transferrin Receptor Monoclonal Antibody (DF1513)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
- Antibody clone number
- DF1513
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Transferrin Receptor was performed using 70% confluent log phase HeLa cells treated with BrefeldinA (5 µg/mL for 30 minutes). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Transferrin Receptor Monoclonal Antibody (Product # MA5-16643) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing accumulation in the golgi network upon BrefeldinA treatment. Panel e represents the control cells showing cytoplasmic staining. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of equine peripheral blood lymphocytes with Mouse anti Equine CD11a/CD18, visualized with Rabbit anti Mouse IgG conjugated to FITC
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using a CD71/transferrin receptor monoclonal antibody (Product # MA5-16643)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MOLT4 (human acute lymphoblastic leukemia) cells using a CD71/transferrin receptor monoclonal antibody (Product # MA5-16643)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of KG1 cells using a CD71/transferrin receptor monoclonal antibody (Product # MA5-16643)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MOLT4 (human acute lymphoblastic leukemia) cells using a CD71/transferrin receptor monoclonal antibody (Product # MA5-16643)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using a CD71/transferrin receptor monoclonal antibody (Product # MA5-16643)