GTX629816

antibody from GeneTex

Targeting: ATG12 APG12, APG12L

Immunocytochemistry

Antibody data

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Validation data

ATG12 protein structure - GTX629816 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT948] (GTX629816) diluted at 1:500.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Prod no: Proprietary.
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
Antibody Lot Number: 41463

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG12 antibody [GT948] detects ATG12 protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract12 % SDS-PAGEATG12 antibody [GT948] (GTX629816) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATG12 antibody [GT948] detects ATG12 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extractB. 30 ?g GL261 whole cell lysate/extractC. 30 ?g C8D30 whole cell lysate/extractD. 30 ?g NIH-3T3 whole cell lysate/extractE. 30 ?g BCL-1 whole cell lysate/extractF. 30 ?g Raw264.7 whole cell lysate/extractG. 30 ?g C2C12 whole cell lysate/extract12 % SDS-PAGEATG12 antibody [GT948] (GTX629816) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT948] (GTX629816) diluted at 1:500.