Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- NBP2-43781 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ATG12 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Adipose tissue conditioned media support macrophage lipid-droplet biogenesis by interfering with autophagic flux.
Bechor S, Nachmias D, Elia N, Haim Y, Vatarescu M, Leikin-Frenkel A, Gericke M, Tarnovscki T, Las G, Rudich A
Biochimica et biophysica acta. Molecular and cell biology of lipids 2017 Sep;1862(9):1001-1012
Biochimica et biophysica acta. Molecular and cell biology of lipids 2017 Sep;1862(9):1001-1012
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract G. 30 ug C2C12 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATG12 Antibody (166) [NBP2-43781] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 12 % SDS-PAGE ATG12 antibody [166] dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATG12 Antibody (166) [NBP2-43781] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [166].