ABIN350097

antibody from antibodies-online

Targeting: ATG12 APG12, APG12L

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN350097

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN350097, RRID:AB_10805657

Product name

anti-Autophagy Related 12 (ATG12) antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide from human ATG12 conjugated to an immunogenic carrier protein has been used as the antigen.

Reactivity

Human, Mouse, Rat

Host

Goat

Isotype

IgG

Vial size

500 μg

Storage

Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability.

Handling

Avoid freeze and thaw cycles.

References

Submitted references

Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12.

Prigione A, Cortopassi G
Autophagy 2007 Jul-Aug;3(4):377-80

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Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate.

Mizushima N, Kuma A, Kobayashi Y, Yamamoto A, Matsubae M, Takao T, Natsume T, Ohsumi Y, Yoshimori T
Journal of cell science 2003 May 1;116(Pt 9):1679-88

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Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing.

Tanida I, Nishitani T, Nemoto T, Ueno T, Kominami E
Biochemical and biophysical research communications 2002 Sep 6;296(5):1164-70

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Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method.

Mizushima N, Yoshimori T, Ohsumi Y
FEBS letters 2002 Dec 18;532(3):450-4

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Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p.

Tanida I, Tanida-Miyake E, Komatsu M, Ueno T, Kominami E
The Journal of biological chemistry 2002 Apr 19;277(16):13739-44