AM20221PU-N

antibody from Acris Antibodies GmbH

Targeting: RPTOR KIAA1303, KOG1, Mip1, raptor

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM20221PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM20221PU-N, RRID:AB_10754112

Product name

anti RAPTOR (incl. pos. control)

Antibody type

Monoclonal

Antigen

Peptide conjugated to hemocyanin derived from Raptor Sequence.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

10E10

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous Raptor: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with AM20221PU-N Raptor antibody (Clone 10E10) at 0.5 µg/ml for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12Lane 7: CMT-93 Lane 8: Neuro 2A Lane 9: 3T3