Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB200-195 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-195, RRID:AB_10002674
- Product name
- Rabbit Polyclonal DDX3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX3 Antibody [NB200-195] - Detection of DDX3 by Western Blot and Immunoprecipitation. Samples: A. Whole cell lysate from HeLa (5, 15 and 50 mcg) and Ramos (50 mcg) cells. B. Whole cell lysate (1 mg/IP; 1/4 of IP loaded/lane) from HeLa cells. Antibodies: NB 200-195 used at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) for Western Blot and at 3 mcg/mg lysate for IP (B). DDX3 was also immunoprecipitated using NB 200-194 and NB 200-196 at 3 mcg/mg lysate. Detection: Chemiluminescence with an exposure time of 10 seconds (A and B).
