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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- MAB4227R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR7/RDC-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCR7/RDC-1 in direct ELISAs. In flow cytometry, reacts specifically with five distinct human CXCR7 transfectants, but does not react with their respective parental lines or mouse CXCR7 transfectants. In flow cytometry, also reacts with monocytes expressing CXCR7, but does not react with MCF-7 cells which have been reported to have surface-expressing CXCR7 using clone 11G8. Due to the conflicting reports published, use of monoclonal MAB4227 may result in an underestimation of CXCR7 expression on certain cell types.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAA62370- Isotype
- IgG
- Antibody clone number
- 358426R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR7/RDC-1 in Human Breast Cancer Tissue. CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227R) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR7/RDC-1 in HEK293 Human Cell Line Transfected with Human CXCR7/RDC-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CXCR7/RDC-1 and eGFP was stained with (A) Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227R) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR7/RDC-1 in Human peripheral blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB4227R, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by APC-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
