PA5-78464

antibody from Invitrogen Antibodies

Targeting: LSM14A C19orf13, DKFZP434D1335, FAM61A, RAP55, RAP55A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78464

Provider

Invitrogen Antibodies

Product name

LSM14A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A549, H1299, HCT116, NT2D1

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LSM14A in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with LSM14A polyclonal antibody (Product # PA5-78464) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LSM14A in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with LSM14A polyclonal antibody (Product # PA5-78464) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LSM14A Polyclonal Antibody detects LSM14A protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LSM14A Polyclonal Antibody (Product # PA5-78464) diluted by 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LSM14A Polyclonal Antibody detects LSM14A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LSM14A Polyclonal Antibody (Product # PA5-78464) diluted by 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LSM14A Polyclonal Antibody detects LSM14A protein at P-body by immunofluorescent analysis. Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LSM14A protein stained by LSM14A Polyclonal Antibody (Product # PA5-78464) diluted at 1:500. Red: alpha Tubulin , a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (Product # MA5-31466) diluted at 1:1,000. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LSM14A Polyclonal Antibody detects LSM14A protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. LSM14A Polyclonal Antibody (Product # PA5-78464) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LSM14A Polyclonal Antibody detects LSM14A protein at cytosol on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. LSM14A Polyclonal Antibody (Product # PA5-78464) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.