NBP2-33297

antibody from Novus Biologicals

Targeting: FOXP3 AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-33297

Provider

Novus Biologicals

Product name

Mouse Monoclonal FoxP3 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FoxP3 Antibody (3G3) [Azide Free] [NBP2-33297] - Analysis in 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: FoxP3 Antibody (3G3) [Azide Free] [NBP2-33297] - Analysis in 2x10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: FoxP3 Antibody (3G3) - Azide Free [NBP2-33297] - FoxP3 Antibody (3G3) [Azide Free] [NBP2-33297] - Analysis in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and NBP2-33297PE (right) at 0.1 ug/10^6 cells. Products used: anti-CD4 FITC conjugate, anti CD25 APC conjugate, anti FOXP3 PE conjugate (NBP2-33297PE).