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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA810542 - Provider product page
- Provider
- OriGene
- Product name
- FOXP3 mouse monoclonal antibody,clone OTI3E11
- Antibody type
- Monoclonal
- Description
- FOXP3 mouse monoclonal antibody,clone OTI3E11
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FOXP3
- Isotype
- IgG
- Antibody clone number
- OTI3E11
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXP3 (RC225614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXP3.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Permeabilized HEK293T cells transfected with either RC225614 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FOXP3 antibody(TA810542), and then analyzed by flow cytometry.(1:100)
- Validation comment
- FC
