14-4777-82
antibody from Invitrogen Antibodies
Targeting: FOXP3
AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID
Antibody data
- Antibody Data
- Antigen structure
- References [132]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [49]
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Validation data
Reference
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- Product number
- 14-4777-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXP3 Monoclonal Antibody (236A/E7), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.
- Antibody clone number
- 236A/E7
- Concentration
- 0.5 mg/mL
Submitted references An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.
Analysis of Expression of Different Histone Deacetylases in Autoimmune Thyroid Disease.
Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma.
Heme Oxygenase-1 Inhibition Potentiates the Effects of Nab-Paclitaxel-Gemcitabine and Modulates the Tumor Microenvironment in Pancreatic Ductal Adenocarcinoma.
Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.
Immune cell composition in the endometrium of patients with a complete molar pregnancy: Effects on outcome.
Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients.
Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.
Increased CD3(+), CD8(+), or FoxP3(+) T Lymphocyte Infiltrations Are Associated with the Pathogenesis of Colorectal Cancer but Not with the Overall Survival of Patients.
Digital Pathology Analysis Quantifies Spatial Heterogeneity of CD3, CD4, CD8, CD20, and FoxP3 Immune Markers in Triple-Negative Breast Cancer.
Clusters of Tolerogenic B Cells Feature in the Dynamic Immunological Landscape of the Pregnant Uterus.
Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs.
Muscle-Directed Delivery of an AAV1 Vector Leads to Capsid-Specific T Cell Exhaustion in Nonhuman Primates and Humans.
Mitochondrial transfer from MSCs to T cells induces Treg differentiation and restricts inflammatory response.
Metformin Abrogates Age-Associated Ovarian Fibrosis.
hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.
Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome.
Inhibition of RANK signaling in breast cancer induces an anti-tumor immune response orchestrated by CD8+ T cells.
Biomarker recommendation for PD-1/PD-L1 immunotherapy development in pediatric cancer based on digital image analysis of PD-L1 and immune cells.
A Four-Chemokine Signature Is Associated with a T-cell-Inflamed Phenotype in Primary and Metastatic Pancreatic Cancer.
Depleting T regulatory cells by targeting intracellular Foxp3 with a TCR mimic antibody.
Molecular profiling of primary uveal melanomas with tumor-infiltrating lymphocytes.
Undernutrition is associated with perturbations in T cell-, B cell-, monocyte- and dendritic cell- subsets in latent Mycobacterium tuberculosis infection.
GARP Dampens Cancer Immunity by Sustaining Function and Accumulation of Regulatory T Cells in the Colon.
Poor Response to Neoadjuvant Chemotherapy Correlates with Mast Cell Infiltration in Inflammatory Breast Cancer.
CD55 Is Essential for CD103(+) Dendritic Cell Tolerogenic Responses that Protect against Autoimmunity.
Immunotherapy Targeting HPV16/18 Generates Potent Immune Responses in HPV-Associated Head and Neck Cancer.
Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy.
Spontaneous Regression of Ovarian Carcinoma After Septic Peritonitis; A Unique Case Report.
Dynamic evaluation of the immune infiltrate and immune function genes as predictive markers for neoadjuvant chemotherapy in hormone receptor positive, HER2 negative breast cancer.
Increased circulating regulatory T cells in medicated people with schizophrenia.
Age Correlates with Response to Anti-PD1, Reflecting Age-Related Differences in Intratumoral Effector and Regulatory T-Cell Populations.
Alternative Splicing of FOXP3 Controls Regulatory T Cell Effector Functions and Is Associated With Human Atherosclerotic Plaque Stability.
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
FOXP3(+) regulatory T cells in normal prostate tissue, postatrophic hyperplasia, prostatic intraepithelial neoplasia, and tumor histological lesions in men with and without prostate cancer.
Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.
Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells.
Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.
PD-L1 expression and the immune microenvironment in primary invasive lobular carcinomas of the breast.
Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.
Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.
Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.
A novel method for assaying human regulatory T cell direct suppression of B cell effector function.
ICOS(+) Foxp3(+) TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.
TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Clinical, Genetic and Innate Immunity Characteristics of Melanoma in Organ Transplant Recipients.
Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression.
An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.
Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways.
Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo.
The ratio of CD8 to Treg tumor-infiltrating lymphocytes is associated with response to cisplatin-based neoadjuvant chemotherapy in patients with muscle invasive urothelial carcinoma of the bladder.
The immune microenvironment of breast ductal carcinoma in situ.
Clinical efficacy of a new CD28-targeting antagonist of T cell co-stimulation in a non-human primate model of collagen-induced arthritis.
Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.
Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.
Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer.
PD-L1 (B7-H1) expression and the immune tumor microenvironment in primary and metastatic breast carcinomas.
Low density of FOXP3-positive T cells in normal colonic mucosa is related to the presence of beta2-microglobulin mutations in Lynch syndrome-associated colorectal cancer.
Fine needle aspirate flow cytometric phenotyping characterizes immunosuppressive nature of the mesothelioma microenvironment.
Follicular B Lymphomas Generate Regulatory T Cells via the ICOS/ICOSL Pathway and Are Susceptible to Treatment by Anti-ICOS/ICOSL Therapy.
Effects of conventional immunosuppressive treatment on CD244+ (CD28null) and FOXP3+ T cells in the inflamed muscle of patients with polymyositis and dermatomyositis.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Changes in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation with JAK2(V617F) allelic burden.
Mismatch repair-deficient crypt foci in Lynch syndrome--molecular alterations and association with clinical parameters.
Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
CD8(+)/FOXP3(+)-ratio in osteosarcoma microenvironment separates survivors from non-survivors: a multicenter validated retrospective study.
Immunoprofiling for prognostic assessment of colon cancer: a novel complement to ultrastaging.
Regulatory T cells, inflammation, and endoplasmic reticulum stress in women with defective endometrial receptivity.
CD4+ and CD8+ T cells have opposing roles in breast cancer progression and outcome.
Early-onset lymphoproliferation and autoimmunity caused by germline STAT3 gain-of-function mutations.
Ectopic lymphoid structures function as microniches for tumor progenitor cells in hepatocellular carcinoma.
Distribution and prognostic relevance of tumor-infiltrating lymphocytes (TILs) and PD-1/PD-L1 immune checkpoints in human brain metastases.
Osteosarcoma microenvironment: whole-slide imaging and optimized antigen detection overcome major limitations in immunohistochemical quantification.
Th1 response and systemic treg deficiency in inclusion body myositis.
Porphyromonas gingivalis infection reduces regulatory T cells in infected atherosclerosis patients.
FOXP3 transcription factor: a candidate marker for susceptibility and prognosis in triple negative breast cancer.
Adoptive TIL transfer in the adjuvant setting for melanoma: long-term patient survival.
Generation and in vivo evaluation of IL10-treated dendritic cells in a nonhuman primate model of AAV-based gene transfer.
Altered expression of autoimmune regulator in infant down syndrome thymus, a possible contributor to an autoimmune phenotype.
Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.
Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.
Selective targeting of Toll-like receptors and OX40 inhibit regulatory T-cell function in follicular lymphoma.
CD4⁺CD25⁻Foxp3⁺ T cells: a marker for lupus nephritis?
Inhibition of CD4+CD25+ regulatory T cell function and conversion into Th1-like effectors by a Toll-like receptor-activated dendritic cell vaccine.
DC-LAMP+ dendritic cells are recruited to gastric lymphoid follicles in Helicobacter pylori-infected individuals.
Metastatic triple-negative breast cancers at first relapse have fewer tumor-infiltrating lymphocytes than their matched primary breast tumors: a pilot study.
CD4 helper T cells, CD8 cytotoxic T cells, and FOXP3(+) regulatory T cells with respect to lethal prostate cancer.
The microenvironment of human neuroblastoma supports the activation of tumor-associated T lymphocytes.
HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.
Tissue biomarkers in melanoma patients treated with TIL.
Decrease in proportion of CD19+ CD24(hi) CD27+ B cells and impairment of their suppressive function in Graves' disease.
Paradoxically increased FOXP3+ T cells in IBD do not preferentially express the isoform of FOXP3 lacking exon 2.
Phenotypic characterization of leukocytes in prenatal human dermis.
Tumor-infiltrating γδ T lymphocytes predict clinical outcome in human breast cancer.
HTLV-1 modulates the frequency and phenotype of FoxP3+CD4+ T cells in virus-infected individuals.
IDO and regulatory T cell support are critical for cytotoxic T lymphocyte-associated Ag-4 Ig-mediated long-term solid organ allograft survival.
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Intracerebral human regulatory T cells: analysis of CD4+ CD25+ FOXP3+ T cells in brain lesions and cerebrospinal fluid of multiple sclerosis patients.
The numbers of FoxP3+ lymphocytes in sentinel lymph nodes of breast cancer patients correlate with primary tumor size but not nodal status.
Immunostaining of PD-1/PD-Ls in liver tissues of patients with hepatitis and hepatocellular carcinoma.
HTLV-1 bZIP factor induces T-cell lymphoma and systemic inflammation in vivo.
Progesterone promotes differentiation of human cord blood fetal T cells into T regulatory cells but suppresses their differentiation into Th17 cells.
A longitudinal, integrated, clinical, histological and mRNA profiling study of resistance exercise in myositis.
Refractory colitis following anti-CTLA4 antibody therapy: analysis of mucosal FOXP3+ T cells.
Recipient NOD2/CARD15 status affects cellular infiltrates in human intestinal graft-versus-host disease.
Inducing CTLA-4-dependent immune regulation by selective CD28 blockade promotes regulatory T cells in organ transplantation.
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
Increased quantity of tumor-infiltrating FOXP3-positive regulatory T cells is an independent predictor for improved clinical outcome in extranodal NK/T-cell lymphoma.
Foxp3 regulates megakaryopoiesis and platelet function.
Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells.
CD49d provides access to "untouched" human Foxp3+ Treg free of contaminating effector cells.
The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells.
Regulatory T cells and the PD-L1/PD-1 pathway mediate immune suppression in malignant human brain tumors.
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
Malignant Tregs express low molecular splice forms of FOXP3 in Sézary syndrome.
Epigenetic inheritance of DNA methylation limits activation-induced expression of FOXP3 in conventional human CD25-CD4+ T cells.
Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes.
Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes.
Foxp3 expression in human cancer cells.
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level.
Outcome in Hodgkin's lymphoma can be predicted from the presence of accompanying cytotoxic and regulatory T cells.
FOXP3, a selective marker for a subset of adult T-cell leukaemia/lymphoma.
The expression of the regulatory T cell-specific forkhead box transcription factor FoxP3 is associated with poor prognosis in ovarian cancer.
CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene.
The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery.
Control of regulatory T cell development by the transcription factor Foxp3.
Johnson BE, Creason AL, Stommel JM, Keck JM, Parmar S, Betts CB, Blucher A, Boniface C, Bucher E, Burlingame E, Camp T, Chin K, Eng J, Estabrook J, Feiler HS, Heskett MB, Hu Z, Kolodzie A, Kong BL, Labrie M, Lee J, Leyshock P, Mitri S, Patterson J, Riesterer JL, Sivagnanam S, Somers J, Sudar D, Thibault G, Weeder BR, Zheng C, Nan X, Thompson RF, Heiser LM, Spellman PT, Thomas G, Demir E, Chang YH, Coussens LM, Guimaraes AR, Corless C, Goecks J, Bergan R, Mitri Z, Mills GB, Gray JW
Cell reports. Medicine 2022 Feb 15;3(2):100525
Cell reports. Medicine 2022 Feb 15;3(2):100525
Analysis of Expression of Different Histone Deacetylases in Autoimmune Thyroid Disease.
Sacristán-Gómez P, Serrano-Somavilla A, González-Amaro R, Martínez-Hernández R, Marazuela M
The Journal of clinical endocrinology and metabolism 2021 Oct 21;106(11):3213-3227
The Journal of clinical endocrinology and metabolism 2021 Oct 21;106(11):3213-3227
Immune cell topography predicts response to PD-1 blockade in cutaneous T cell lymphoma.
Phillips D, Matusiak M, Gutierrez BR, Bhate SS, Barlow GL, Jiang S, Demeter J, Smythe KS, Pierce RH, Fling SP, Ramchurren N, Cheever MA, Goltsev Y, West RB, Khodadoust MS, Kim YH, Schürch CM, Nolan GP
Nature communications 2021 Nov 18;12(1):6726
Nature communications 2021 Nov 18;12(1):6726
Heme Oxygenase-1 Inhibition Potentiates the Effects of Nab-Paclitaxel-Gemcitabine and Modulates the Tumor Microenvironment in Pancreatic Ductal Adenocarcinoma.
Ahmad IM, Dafferner AJ, O'Connell KA, Mehla K, Britigan BE, Hollingsworth MA, Abdalla MY
Cancers 2021 May 8;13(9)
Cancers 2021 May 8;13(9)
Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.
Schwabenland M, Salié H, Tanevski J, Killmer S, Lago MS, Schlaak AE, Mayer L, Matschke J, Püschel K, Fitzek A, Ondruschka B, Mei HE, Boettler T, Neumann-Haefelin C, Hofmann M, Breithaupt A, Genc N, Stadelmann C, Saez-Rodriguez J, Bronsert P, Knobeloch KP, Blank T, Thimme R, Glatzel M, Prinz M, Bengsch B
Immunity 2021 Jul 13;54(7):1594-1610.e11
Immunity 2021 Jul 13;54(7):1594-1610.e11
Immune cell composition in the endometrium of patients with a complete molar pregnancy: Effects on outcome.
Hoeijmakers YM, Gorris MAJ, Sweep FCGJ, Bulten J, Eysbouts YK, Massuger LFAG, Ottevanger PB, de Vries JIJM
Gynecologic oncology 2021 Feb;160(2):450-456
Gynecologic oncology 2021 Feb;160(2):450-456
Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients.
Xydia M, Rahbari R, Ruggiero E, Macaulay I, Tarabichi M, Lohmayer R, Wilkening S, Michels T, Brown D, Vanuytven S, Mastitskaya S, Laidlaw S, Grabe N, Pritsch M, Fronza R, Hexel K, Schmitt S, Müller-Steinhardt M, Halama N, Domschke C, Schmidt M, von Kalle C, Schütz F, Voet T, Beckhove P
Nature communications 2021 Feb 18;12(1):1119
Nature communications 2021 Feb 18;12(1):1119
Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.
von Schoenfeld A, Bronsert P, Poc M, Fuller A, Filby A, Kraft S, Kurowski K, Sörensen K, Huber J, Pfeiffer J, Proietti M, Stehl V, Werner M, Seidl M
International journal of molecular sciences 2021 Dec 25;23(1)
International journal of molecular sciences 2021 Dec 25;23(1)
Increased CD3(+), CD8(+), or FoxP3(+) T Lymphocyte Infiltrations Are Associated with the Pathogenesis of Colorectal Cancer but Not with the Overall Survival of Patients.
Barbosa AM, Martinho O, Nogueira R, Campos J, Lobo L, Pinto H, Longatto-Filho A, Castro AG, Martins SF, Torrado E
Biology 2021 Aug 20;10(8)
Biology 2021 Aug 20;10(8)
Digital Pathology Analysis Quantifies Spatial Heterogeneity of CD3, CD4, CD8, CD20, and FoxP3 Immune Markers in Triple-Negative Breast Cancer.
Mi H, Gong C, Sulam J, Fertig EJ, Szalay AS, Jaffee EM, Stearns V, Emens LA, Cimino-Mathews AM, Popel AS
Frontiers in physiology 2020;11:583333
Frontiers in physiology 2020;11:583333
Clusters of Tolerogenic B Cells Feature in the Dynamic Immunological Landscape of the Pregnant Uterus.
Benner M, Feyaerts D, García CC, Inci N, López SC, Fasse E, Shadmanfar W, van der Heijden OWH, Gorris MAJ, Joosten I, Ferwerda G, van der Molen RG
Cell reports 2020 Sep 29;32(13):108204
Cell reports 2020 Sep 29;32(13):108204
Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs.
Scherer A, Stephens VR, McGivney GR, Gutierrez WR, Laverty EA, Knepper-Adrian V, Dodd RD
Genes 2020 May 23;11(5)
Genes 2020 May 23;11(5)
Muscle-Directed Delivery of an AAV1 Vector Leads to Capsid-Specific T Cell Exhaustion in Nonhuman Primates and Humans.
Gernoux G, Gruntman AM, Blackwood M, Zieger M, Flotte TR, Mueller C
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Mar 4;28(3):747-757
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Mar 4;28(3):747-757
Mitochondrial transfer from MSCs to T cells induces Treg differentiation and restricts inflammatory response.
Court AC, Le-Gatt A, Luz-Crawford P, Parra E, Aliaga-Tobar V, Bátiz LF, Contreras RA, Ortúzar MI, Kurte M, Elizondo-Vega R, Maracaja-Coutinho V, Pino-Lagos K, Figueroa FE, Khoury M
EMBO reports 2020 Feb 5;21(2):e48052
EMBO reports 2020 Feb 5;21(2):e48052
Metformin Abrogates Age-Associated Ovarian Fibrosis.
McCloskey CW, Cook DP, Kelly BS, Azzi F, Allen CH, Forsyth A, Upham J, Rayner KJ, Gray DA, Boyd RW, Murugkar S, Lo B, Trudel D, Senterman MK, Vanderhyden BC
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Feb 1;26(3):632-642
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Feb 1;26(3):632-642
hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts.
Todorova D, Zhang Y, Chen Q, Liu J, He J, Fu X, Xu Y
EBioMedicine 2020 Dec;62:103120
EBioMedicine 2020 Dec;62:103120
Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome.
Zhang M, Liu Y, Chen J, Chen L, Meng J, Yang C, Yin S, Zhang X, Zhang L, Hao Z, Chen X, Liang C
Journal of cellular and molecular medicine 2020 Dec;24(23):14099-14109
Journal of cellular and molecular medicine 2020 Dec;24(23):14099-14109
Inhibition of RANK signaling in breast cancer induces an anti-tumor immune response orchestrated by CD8+ T cells.
Gómez-Aleza C, Nguyen B, Yoldi G, Ciscar M, Barranco A, Hernández-Jiménez E, Maetens M, Salgado R, Zafeiroglou M, Pellegrini P, Venet D, Garaud S, Trinidad EM, Benítez S, Vuylsteke P, Polastro L, Wildiers H, Simon P, Lindeman G, Larsimont D, Van den Eynden G, Velghe C, Rothé F, Willard-Gallo K, Michiels S, Muñoz P, Walzer T, Planelles L, Penninger J, Azim HA Jr, Loi S, Piccart M, Sotiriou C, González-Suárez E
Nature communications 2020 Dec 10;11(1):6335
Nature communications 2020 Dec 10;11(1):6335
Biomarker recommendation for PD-1/PD-L1 immunotherapy development in pediatric cancer based on digital image analysis of PD-L1 and immune cells.
Silva MA, Triltsch N, Leis S, Kanchev I, Tan TH, Van Peel B, Van Kerckhoven M, Deschoolmeester V, Zimmermann J
The journal of pathology. Clinical research 2020 Apr;6(2):124-137
The journal of pathology. Clinical research 2020 Apr;6(2):124-137
A Four-Chemokine Signature Is Associated with a T-cell-Inflamed Phenotype in Primary and Metastatic Pancreatic Cancer.
Romero JM, Grünwald B, Jang GH, Bavi PP, Jhaveri A, Masoomian M, Fischer SE, Zhang A, Denroche RE, Lungu IM, De Luca A, Bartlett JMS, Xu J, Li N, Dhaliwal S, Liang SB, Chadwick D, Vyas F, Bronsert P, Khokha R, McGaha TL, Notta F, Ohashi PS, Done SJ, O'Kane GM, Wilson JM, Knox JJ, Connor A, Wang Y, Zogopoulos G, Gallinger S
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 15;26(8):1997-2010
Clinical cancer research : an official journal of the American Association for Cancer Research 2020 Apr 15;26(8):1997-2010
Depleting T regulatory cells by targeting intracellular Foxp3 with a TCR mimic antibody.
Dao T, Mun SS, Scott AC, Jarvis CA, Korontsvit T, Yang Z, Liu L, Klatt MG, Guerreiro M, Selvakumar A, Brea EJ, Oh C, Liu C, Scheinberg DA
Oncoimmunology 2019;8(7):1570778
Oncoimmunology 2019;8(7):1570778
Molecular profiling of primary uveal melanomas with tumor-infiltrating lymphocytes.
Triozzi PL, Schoenfield L, Plesec T, Saunthararajah Y, Tubbs RR, Singh AD
Oncoimmunology 2019;8(10):e947169
Oncoimmunology 2019;8(10):e947169
Undernutrition is associated with perturbations in T cell-, B cell-, monocyte- and dendritic cell- subsets in latent Mycobacterium tuberculosis infection.
Rajamanickam A, Munisankar S, Dolla CK, Babu S
PloS one 2019;14(12):e0225611
PloS one 2019;14(12):e0225611
GARP Dampens Cancer Immunity by Sustaining Function and Accumulation of Regulatory T Cells in the Colon.
Salem M, Wallace C, Velegraki M, Li A, Ansa-Addo E, Metelli A, Kwon H, Riesenberg B, Wu B, Zhang Y, Guglietta S, Sun S, Liu B, Li Z
Cancer research 2019 Mar 15;79(6):1178-1190
Cancer research 2019 Mar 15;79(6):1178-1190
Poor Response to Neoadjuvant Chemotherapy Correlates with Mast Cell Infiltration in Inflammatory Breast Cancer.
Reddy SM, Reuben A, Barua S, Jiang H, Zhang S, Wang L, Gopalakrishnan V, Hudgens CW, Tetzlaff MT, Reuben JM, Tsujikawa T, Coussens LM, Wani K, He Y, Villareal L, Wood A, Rao A, Woodward WA, Ueno NT, Krishnamurthy S, Wargo JA, Mittendorf EA
Cancer immunology research 2019 Jun;7(6):1025-1035
Cancer immunology research 2019 Jun;7(6):1025-1035
CD55 Is Essential for CD103(+) Dendritic Cell Tolerogenic Responses that Protect against Autoimmunity.
Strainic MG, Liu J, An F, Bailey E, Esposito A, Hamann J, Heeger PS, Medof ME
The American journal of pathology 2019 Jul;189(7):1386-1401
The American journal of pathology 2019 Jul;189(7):1386-1401
Immunotherapy Targeting HPV16/18 Generates Potent Immune Responses in HPV-Associated Head and Neck Cancer.
Aggarwal C, Cohen RB, Morrow MP, Kraynyak KA, Sylvester AJ, Knoblock DM, Bauml JM, Weinstein GS, Lin A, Boyer J, Sakata L, Tan S, Anton A, Dickerson K, Mangrolia D, Vang R, Dallas M, Oyola S, Duff S, Esser M, Kumar R, Weiner D, Csiki I, Bagarazzi ML
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Jan 1;25(1):110-124
Clinical cancer research : an official journal of the American Association for Cancer Research 2019 Jan 1;25(1):110-124
Hijacking antibody-induced CTLA-4 lysosomal degradation for safer and more effective cancer immunotherapy.
Zhang Y, Du X, Liu M, Tang F, Zhang P, Ai C, Fields JK, Sundberg EJ, Latinovic OS, Devenport M, Zheng P, Liu Y
Cell research 2019 Aug;29(8):609-627
Cell research 2019 Aug;29(8):609-627
Spontaneous Regression of Ovarian Carcinoma After Septic Peritonitis; A Unique Case Report.
Roelofsen T, Wefers C, Gorris MAJ, Textor JC, Massuger LFAG, de Vries IJM, van Altena AM
Frontiers in oncology 2018;8:562
Frontiers in oncology 2018;8:562
Dynamic evaluation of the immune infiltrate and immune function genes as predictive markers for neoadjuvant chemotherapy in hormone receptor positive, HER2 negative breast cancer.
Matikas A, Lövrot J, Ramberg A, Eriksson M, Lindsten T, Lekberg T, Hedenfalk I, Loman N, Bergh J, Hatschek T, Erlandsson A, Foukakis T
Oncoimmunology 2018;7(9):e1466017
Oncoimmunology 2018;7(9):e1466017
Increased circulating regulatory T cells in medicated people with schizophrenia.
Kelly DL, Li X, Kilday C, Feldman S, Clark S, Liu F, Buchanan RW, Tonelli LH
Psychiatry research 2018 Nov;269:517-523
Psychiatry research 2018 Nov;269:517-523
Age Correlates with Response to Anti-PD1, Reflecting Age-Related Differences in Intratumoral Effector and Regulatory T-Cell Populations.
Kugel CH 3rd, Douglass SM, Webster MR, Kaur A, Liu Q, Yin X, Weiss SA, Darvishian F, Al-Rohil RN, Ndoye A, Behera R, Alicea GM, Ecker BL, Fane M, Allegrezza MJ, Svoronos N, Kumar V, Wang DY, Somasundaram R, Hu-Lieskovan S, Ozgun A, Herlyn M, Conejo-Garcia JR, Gabrilovich D, Stone EL, Nowicki TS, Sosman J, Rai R, Carlino MS, Long GV, Marais R, Ribas A, Eroglu Z, Davies MA, Schilling B, Schadendorf D, Xu W, Amaravadi RK, Menzies AM, McQuade JL, Johnson DB, Osman I, Weeraratna AT
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Nov 1;24(21):5347-5356
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Nov 1;24(21):5347-5356
Alternative Splicing of FOXP3 Controls Regulatory T Cell Effector Functions and Is Associated With Human Atherosclerotic Plaque Stability.
Joly AL, Seitz C, Liu S, Kuznetsov NV, Gertow K, Westerberg LS, Paulsson-Berne G, Hansson GK, Andersson J
Circulation research 2018 May 11;122(10):1385-1394
Circulation research 2018 May 11;122(10):1385-1394
Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.
Seki A, Rutz S
The Journal of experimental medicine 2018 Mar 5;215(3):985-997
The Journal of experimental medicine 2018 Mar 5;215(3):985-997
FOXP3(+) regulatory T cells in normal prostate tissue, postatrophic hyperplasia, prostatic intraepithelial neoplasia, and tumor histological lesions in men with and without prostate cancer.
Davidsson S, Andren O, Ohlson AL, Carlsson J, Andersson SO, Giunchi F, Rider JR, Fiorentino M
The Prostate 2018 Jan;78(1):40-47
The Prostate 2018 Jan;78(1):40-47
Multicomponent analysis of the tumour microenvironment reveals low CD8 T cell number, low stromal caveolin-1 and high tenascin-C and their combination as significant prognostic markers in non-small cell lung cancer.
Onion D, Isherwood M, Shridhar N, Xenophontos M, Craze ML, Day LJ, García-Márquez MA, Pineda RG, Reece-Smith AM, Saunders JH, Duffy JP, Argent RH, Grabowska AM
Oncotarget 2018 Jan 5;9(2):1760-1771
Oncotarget 2018 Jan 5;9(2):1760-1771
Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells.
Schulten V, Tripple V, Seumois G, Qian Y, Scheuermann RH, Fu Z, Locci M, Rosales S, Vijayanand P, Sette A, Alam R, Crotty S, Peters B
The Journal of allergy and clinical immunology 2018 Feb;141(2):775-777.e6
The Journal of allergy and clinical immunology 2018 Feb;141(2):775-777.e6
Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.
Jiang X, Seo YD, Chang JH, Coveler A, Nigjeh EN, Pan S, Jalikis F, Yeung RS, Crispe IN, Pillarisetty VG
Oncoimmunology 2017;6(7):e1333210
Oncoimmunology 2017;6(7):e1333210
PD-L1 expression and the immune microenvironment in primary invasive lobular carcinomas of the breast.
Thompson ED, Taube JM, Asch-Kendrick RJ, Ogurtsova A, Xu H, Sharma R, Meeker A, Argani P, Emens LA, Cimino-Mathews A
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2017 Nov;30(11):1551-1560
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2017 Nov;30(11):1551-1560
Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.
Kishore M, Cheung KCP, Fu H, Bonacina F, Wang G, Coe D, Ward EJ, Colamatteo A, Jangani M, Baragetti A, Matarese G, Smith DM, Haas R, Mauro C, Wraith DC, Okkenhaug K, Catapano AL, De Rosa V, Norata GD, Marelli-Berg FM
Immunity 2017 Nov 21;47(5):875-889.e10
Immunity 2017 Nov 21;47(5):875-889.e10
Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.
Syed Khaja AS, Toor SM, El Salhat H, Faour I, Ul Haq N, Ali BR, Elkord E
Oncotarget 2017 May 16;8(20):33159-33171
Oncotarget 2017 May 16;8(20):33159-33171
Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.
Zanin-Zhorov A, Weiss JM, Trzeciak A, Chen W, Zhang J, Nyuydzefe MS, Arencibia C, Polimera S, Schueller O, Fuentes-Duculan J, Bonifacio KM, Kunjravia N, Cueto I, Soung J, Fleischmann RM, Kivitz A, Lebwohl M, Nunez M, Woodson J, Smith SL, West RF, Berger M, Krueger JG, Ryan JL, Waksal SD
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3809-3814
Journal of immunology (Baltimore, Md. : 1950) 2017 May 15;198(10):3809-3814
A novel method for assaying human regulatory T cell direct suppression of B cell effector function.
Weingartner E, Courneya JP, Keegan A, Golding A
Journal of immunological methods 2017 Feb;441:1-7
Journal of immunological methods 2017 Feb;441:1-7
ICOS(+) Foxp3(+) TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.
Nagase H, Takeoka T, Urakawa S, Morimoto-Okazawa A, Kawashima A, Iwahori K, Takiguchi S, Nishikawa H, Sato E, Sakaguchi S, Mori M, Doki Y, Wada H
International journal of cancer 2017 Feb 1;140(3):686-695
International journal of cancer 2017 Feb 1;140(3):686-695
TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.
Huang SC, Wei PC, Hwang-Verslues WW, Kuo WH, Jeng YM, Hu CM, Shew JY, Huang CS, Chang KJ, Lee EY, Lee WH
EMBO molecular medicine 2017 Dec;9(12):1660-1680
EMBO molecular medicine 2017 Dec;9(12):1660-1680
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Terranova-Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, Rosenblum MD, Budillon A, Munster PN
Oncotarget 2017 Dec 26;8(69):114156-114172
Oncotarget 2017 Dec 26;8(69):114156-114172
Microenvironment inflammatory infiltrate drives growth speed and outcome of hepatocellular carcinoma: a prospective clinical study.
Critelli R, Milosa F, Faillaci F, Condello R, Turola E, Marzi L, Lei B, Dituri F, Andreani S, Sighinolfi P, Manni P, Maiorana A, Caporali C, di Benedetto F, Del Buono M, De Maria N, Schepis F, Martinez-Chantar ML, Giannelli G, Villa E
Cell death & disease 2017 Aug 24;8(8):e3017
Cell death & disease 2017 Aug 24;8(8):e3017
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Clinical, Genetic and Innate Immunity Characteristics of Melanoma in Organ Transplant Recipients.
Brocard A, Knol AC, Bossard C, Denis MG, Quéreux G, Saint-Jean M, Peuvrel L, Khammari A, Blancho G, Dantal J, Dréno B
Acta dermato-venereologica 2017 Apr 6;97(4):483-488
Acta dermato-venereologica 2017 Apr 6;97(4):483-488
Inhibition of the JAK/STAT Signaling Pathway in Regulatory T Cells Reveals a Very Dynamic Regulation of Foxp3 Expression.
Goldstein JD, Burlion A, Zaragoza B, Sendeyo K, Polansky JK, Huehn J, Piaggio E, Salomon BL, Marodon G
PloS one 2016;11(4):e0153682
PloS one 2016;11(4):e0153682
An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.
Massoud AH, Charbonnier LM, Lopez D, Pellegrini M, Phipatanakul W, Chatila TA
Nature medicine 2016 Sep;22(9):1013-22
Nature medicine 2016 Sep;22(9):1013-22
Selective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways.
Zwang NA, Zhang R, Germana S, Fan MY, Hastings WD, Cao A, Turka LA
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2016 Sep;16(9):2624-38
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2016 Sep;16(9):2624-38
Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo.
Fischer A, Zundler S, Atreya R, Rath T, Voskens C, Hirschmann S, López-Posadas R, Watson A, Becker C, Schuler G, Neufert C, Atreya I, Neurath MF
Gut 2016 Oct;65(10):1642-64
Gut 2016 Oct;65(10):1642-64
The ratio of CD8 to Treg tumor-infiltrating lymphocytes is associated with response to cisplatin-based neoadjuvant chemotherapy in patients with muscle invasive urothelial carcinoma of the bladder.
Baras AS, Drake C, Liu JJ, Gandhi N, Kates M, Hoque MO, Meeker A, Hahn N, Taube JM, Schoenberg MP, Netto G, Bivalacqua TJ
Oncoimmunology 2016 May;5(5):e1134412
Oncoimmunology 2016 May;5(5):e1134412
The immune microenvironment of breast ductal carcinoma in situ.
Thompson E, Taube JM, Elwood H, Sharma R, Meeker A, Warzecha HN, Argani P, Cimino-Mathews A, Emens LA
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 Mar;29(3):249-58
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 Mar;29(3):249-58
Clinical efficacy of a new CD28-targeting antagonist of T cell co-stimulation in a non-human primate model of collagen-induced arthritis.
Vierboom MP, Breedveld E, Kap YS, Mary C, Poirier N, 't Hart BA, Vanhove B
Clinical and experimental immunology 2016 Mar;183(3):405-18
Clinical and experimental immunology 2016 Mar;183(3):405-18
Regulatory T Cell Modulation by CBP/EP300 Bromodomain Inhibition.
Ghosh S, Taylor A, Chin M, Huang HR, Conery AR, Mertz JA, Salmeron A, Dakle PJ, Mele D, Cote A, Jayaram H, Setser JW, Poy F, Hatzivassiliou G, DeAlmeida-Nagata D, Sandy P, Hatton C, Romero FA, Chiang E, Reimer T, Crawford T, Pardo E, Watson VG, Tsui V, Cochran AG, Zawadzke L, Harmange JC, Audia JE, Bryant BM, Cummings RT, Magnuson SR, Grogan JL, Bellon SF, Albrecht BK, Sims RJ 3rd, Lora JM
The Journal of biological chemistry 2016 Jun 17;291(25):13014-27
The Journal of biological chemistry 2016 Jun 17;291(25):13014-27
Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis.
Liang DY, Hou YQ, Luo LJ, Ao L
International journal of molecular medicine 2016 Jul;38(1):131-8
International journal of molecular medicine 2016 Jul;38(1):131-8
Differential tumor infiltration by T-cells characterizes intrinsic molecular subtypes in breast cancer.
Miyan M, Schmidt-Mende J, Kiessling R, Poschke I, de Boniface J
Journal of translational medicine 2016 Jul 29;14(1):227
Journal of translational medicine 2016 Jul 29;14(1):227
PD-L1 (B7-H1) expression and the immune tumor microenvironment in primary and metastatic breast carcinomas.
Cimino-Mathews A, Thompson E, Taube JM, Ye X, Lu Y, Meeker A, Xu H, Sharma R, Lecksell K, Cornish TC, Cuka N, Argani P, Emens LA
Human pathology 2016 Jan;47(1):52-63
Human pathology 2016 Jan;47(1):52-63
Low density of FOXP3-positive T cells in normal colonic mucosa is related to the presence of beta2-microglobulin mutations in Lynch syndrome-associated colorectal cancer.
Echterdiek F, Janikovits J, Staffa L, Müller M, Lahrmann B, Frühschütz M, Hartog B, Nelius N, Benner A, Tariverdian M, von Knebel Doeberitz M, Grabe N, Kloor M
Oncoimmunology 2016 Feb;5(2):e1075692
Oncoimmunology 2016 Feb;5(2):e1075692
Fine needle aspirate flow cytometric phenotyping characterizes immunosuppressive nature of the mesothelioma microenvironment.
Lizotte PH, Jones RE, Keogh L, Ivanova E, Liu H, Awad MM, Hammerman PS, Gill RR, Richards WG, Barbie DA, Bass AJ, Bueno R, English JM, Bittinger M, Wong KK
Scientific reports 2016 Aug 19;6:31745
Scientific reports 2016 Aug 19;6:31745
Follicular B Lymphomas Generate Regulatory T Cells via the ICOS/ICOSL Pathway and Are Susceptible to Treatment by Anti-ICOS/ICOSL Therapy.
Le KS, Thibult ML, Just-Landi S, Pastor S, Gondois-Rey F, Granjeaud S, Broussais F, Bouabdallah R, Colisson R, Caux C, Ménétrier-Caux C, Leroux D, Xerri L, Olive D
Cancer research 2016 Aug 15;76(16):4648-60
Cancer research 2016 Aug 15;76(16):4648-60
Effects of conventional immunosuppressive treatment on CD244+ (CD28null) and FOXP3+ T cells in the inflamed muscle of patients with polymyositis and dermatomyositis.
Pandya JM, Loell I, Hossain MS, Zong M, Alexanderson H, Raghavan S, Lundberg IE, Malmström V
Arthritis research & therapy 2016 Apr 1;18:80
Arthritis research & therapy 2016 Apr 1;18:80
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
Changes in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation with JAK2(V617F) allelic burden.
Kovacsovics-Bankowski M, Kelley TW, Efimova O, Kim SJ, Wilson A, Swierczek S, Prchal J
Experimental hematology & oncology 2015;5:28
Experimental hematology & oncology 2015;5:28
Mismatch repair-deficient crypt foci in Lynch syndrome--molecular alterations and association with clinical parameters.
Staffa L, Echterdiek F, Nelius N, Benner A, Werft W, Lahrmann B, Grabe N, Schneider M, Tariverdian M, von Knebel Doeberitz M, Bläker H, Kloor M
PloS one 2015;10(3):e0121980
PloS one 2015;10(3):e0121980
Tumor-Infiltrating Immune Cells Are Associated With Prognosis of Gastric Cancer.
Liu K, Yang K, Wu B, Chen H, Chen X, Chen X, Jiang L, Ye F, He D, Lu Z, Xue L, Zhang W, Li Q, Zhou Z, Mo X, Hu J
Medicine 2015 Sep;94(39):e1631
Medicine 2015 Sep;94(39):e1631
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T, McCarter MD, Levy DN, Rakasz EG, Skinner PJ, Connick E
Nature communications 2015 Oct 20;6:8608
Nature communications 2015 Oct 20;6:8608
CD8(+)/FOXP3(+)-ratio in osteosarcoma microenvironment separates survivors from non-survivors: a multicenter validated retrospective study.
Fritzsching B, Fellenberg J, Moskovszky L, Sápi Z, Krenacs T, Machado I, Poeschl J, Lehner B, Szendrõi M, Bosch AL, Bernd L, Csóka M, Mechtersheimer G, Ewerbeck V, Kinscherf R, Kunz P
Oncoimmunology 2015 Mar;4(3):e990800
Oncoimmunology 2015 Mar;4(3):e990800
Immunoprofiling for prognostic assessment of colon cancer: a novel complement to ultrastaging.
Lavotshkin S, Jalas JR, Torisu-Itakura H, Ozao-Choy J, Lee JH, Sim MS, Stojadinovic A, Wainberg Z, Bifulco CB, Fox BA, Bilchik AJ
Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract 2015 Jun;19(6):999-1006
Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract 2015 Jun;19(6):999-1006
Regulatory T cells, inflammation, and endoplasmic reticulum stress in women with defective endometrial receptivity.
Galgani M, Insabato L, Calì G, Della Gatta AN, Mirra P, Papaccio F, Santopaolo M, Alviggi C, Mollo A, Strina I, Matarese G, Beguinot F, De Placido G, Ulianich L
Fertility and sterility 2015 Jun;103(6):1579-86.e1
Fertility and sterility 2015 Jun;103(6):1579-86.e1
CD4+ and CD8+ T cells have opposing roles in breast cancer progression and outcome.
Huang Y, Ma C, Zhang Q, Ye J, Wang F, Zhang Y, Hunborg P, Varvares MA, Hoft DF, Hsueh EC, Peng G
Oncotarget 2015 Jul 10;6(19):17462-78
Oncotarget 2015 Jul 10;6(19):17462-78
Early-onset lymphoproliferation and autoimmunity caused by germline STAT3 gain-of-function mutations.
Milner JD, Vogel TP, Forbes L, Ma CA, Stray-Pedersen A, Niemela JE, Lyons JJ, Engelhardt KR, Zhang Y, Topcagic N, Roberson ED, Matthews H, Verbsky JW, Dasu T, Vargas-Hernandez A, Varghese N, McClain KL, Karam LB, Nahmod K, Makedonas G, Mace EM, Sorte HS, Perminow G, Rao VK, O'Connell MP, Price S, Su HC, Butrick M, McElwee J, Hughes JD, Willet J, Swan D, Xu Y, Santibanez-Koref M, Slowik V, Dinwiddie DL, Ciaccio CE, Saunders CJ, Septer S, Kingsmore SF, White AJ, Cant AJ, Hambleton S, Cooper MA
Blood 2015 Jan 22;125(4):591-9
Blood 2015 Jan 22;125(4):591-9
Ectopic lymphoid structures function as microniches for tumor progenitor cells in hepatocellular carcinoma.
Finkin S, Yuan D, Stein I, Taniguchi K, Weber A, Unger K, Browning JL, Goossens N, Nakagawa S, Gunasekaran G, Schwartz ME, Kobayashi M, Kumada H, Berger M, Pappo O, Rajewsky K, Hoshida Y, Karin M, Heikenwalder M, Ben-Neriah Y, Pikarsky E
Nature immunology 2015 Dec;16(12):1235-44
Nature immunology 2015 Dec;16(12):1235-44
Distribution and prognostic relevance of tumor-infiltrating lymphocytes (TILs) and PD-1/PD-L1 immune checkpoints in human brain metastases.
Harter PN, Bernatz S, Scholz A, Zeiner PS, Zinke J, Kiyose M, Blasel S, Beschorner R, Senft C, Bender B, Ronellenfitsch MW, Wikman H, Glatzel M, Meinhardt M, Juratli TA, Steinbach JP, Plate KH, Wischhusen J, Weide B, Mittelbronn M
Oncotarget 2015 Dec 1;6(38):40836-49
Oncotarget 2015 Dec 1;6(38):40836-49
Osteosarcoma microenvironment: whole-slide imaging and optimized antigen detection overcome major limitations in immunohistochemical quantification.
Kunz P, Fellenberg J, Moskovszky L, Sápi Z, Krenacs T, Poeschl J, Lehner B, Szendrõi M, Ewerbeck V, Kinscherf R, Fritzsching B
PloS one 2014;9(3):e90727
PloS one 2014;9(3):e90727
Th1 response and systemic treg deficiency in inclusion body myositis.
Allenbach Y, Chaara W, Rosenzwajg M, Six A, Prevel N, Mingozzi F, Wanschitz J, Musset L, Charuel JL, Eymard B, Salomon B, Duyckaerts C, Maisonobe T, Dubourg O, Herson S, Klatzmann D, Benveniste O
PloS one 2014;9(3):e88788
PloS one 2014;9(3):e88788
Porphyromonas gingivalis infection reduces regulatory T cells in infected atherosclerosis patients.
Yang J, Wu J, Liu Y, Huang J, Lu Z, Xie L, Sun W, Ji Y
PloS one 2014;9(1):e86599
PloS one 2014;9(1):e86599
FOXP3 transcription factor: a candidate marker for susceptibility and prognosis in triple negative breast cancer.
Lopes LF, Guembarovski RL, Guembarovski AL, Kishima MO, Campos CZ, Oda JM, Ariza CB, de Oliveira KB, Borelli SD, Watanabe MA
BioMed research international 2014;2014:341654
BioMed research international 2014;2014:341654
Adoptive TIL transfer in the adjuvant setting for melanoma: long-term patient survival.
Khammari A, Knol AC, Nguyen JM, Bossard C, Denis MG, Pandolfino MC, Quéreux G, Bercegeay S, Dréno B
Journal of immunology research 2014;2014:186212
Journal of immunology research 2014;2014:186212
Generation and in vivo evaluation of IL10-treated dendritic cells in a nonhuman primate model of AAV-based gene transfer.
Moreau A, Vandamme C, Segovia M, Devaux M, Guilbaud M, Tilly G, Jaulin N, Le Duff J, Cherel Y, Deschamps JY, Anegon I, Moullier P, Cuturi MC, Adjali O
Molecular therapy. Methods & clinical development 2014;1:14028
Molecular therapy. Methods & clinical development 2014;1:14028
Altered expression of autoimmune regulator in infant down syndrome thymus, a possible contributor to an autoimmune phenotype.
Skogberg G, Lundberg V, Lindgren S, Gudmundsdottir J, Sandström K, Kämpe O, Annerén G, Gustafsson J, Sunnegårdh J, van der Post S, Telemo E, Berglund M, Ekwall O
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 1;193(5):2187-95
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 1;193(5):2187-95
Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.
Ye SB, Li ZL, Luo DH, Huang BJ, Chen YS, Zhang XS, Cui J, Zeng YX, Li J
Oncotarget 2014 Jul 30;5(14):5439-52
Oncotarget 2014 Jul 30;5(14):5439-52
Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations.
Schubert D, Bode C, Kenefeck R, Hou TZ, Wing JB, Kennedy A, Bulashevska A, Petersen BS, Schäffer AA, Grüning BA, Unger S, Frede N, Baumann U, Witte T, Schmidt RE, Dueckers G, Niehues T, Seneviratne S, Kanariou M, Speckmann C, Ehl S, Rensing-Ehl A, Warnatz K, Rakhmanov M, Thimme R, Hasselblatt P, Emmerich F, Cathomen T, Backofen R, Fisch P, Seidl M, May A, Schmitt-Graeff A, Ikemizu S, Salzer U, Franke A, Sakaguchi S, Walker LSK, Sansom DM, Grimbacher B
Nature medicine 2014 Dec;20(12):1410-1416
Nature medicine 2014 Dec;20(12):1410-1416
Selective targeting of Toll-like receptors and OX40 inhibit regulatory T-cell function in follicular lymphoma.
Voo KS, Foglietta M, Percivalle E, Chu F, Nattamai D, Harline M, Lee ST, Bover L, Lin HY, Baladandayuthapani V, Delgado D, Luong A, Davis RE, Kwak LW, Liu YJ, Neelapu SS
International journal of cancer 2014 Dec 15;135(12):2834-46
International journal of cancer 2014 Dec 15;135(12):2834-46
CD4⁺CD25⁻Foxp3⁺ T cells: a marker for lupus nephritis?
Bonelli M, Göschl L, Blüml S, Karonitsch T, Steiner CW, Steiner G, Smolen JS, Scheinecker C
Arthritis research & therapy 2014 Apr 28;16(2):R104
Arthritis research & therapy 2014 Apr 28;16(2):R104
Inhibition of CD4+CD25+ regulatory T cell function and conversion into Th1-like effectors by a Toll-like receptor-activated dendritic cell vaccine.
Lee MK 4th, Xu S, Fitzpatrick EH, Sharma A, Graves HL, Czerniecki BJ
PloS one 2013;8(11):e74698
PloS one 2013;8(11):e74698
DC-LAMP+ dendritic cells are recruited to gastric lymphoid follicles in Helicobacter pylori-infected individuals.
Hansson M, Sundquist M, Hering S, Lundin BS, Hermansson M, Quiding-Järbrink M
Infection and immunity 2013 Oct;81(10):3684-92
Infection and immunity 2013 Oct;81(10):3684-92
Metastatic triple-negative breast cancers at first relapse have fewer tumor-infiltrating lymphocytes than their matched primary breast tumors: a pilot study.
Cimino-Mathews A, Ye X, Meeker A, Argani P, Emens LA
Human pathology 2013 Oct;44(10):2055-63
Human pathology 2013 Oct;44(10):2055-63
CD4 helper T cells, CD8 cytotoxic T cells, and FOXP3(+) regulatory T cells with respect to lethal prostate cancer.
Davidsson S, Ohlson AL, Andersson SO, Fall K, Meisner A, Fiorentino M, Andrén O, Rider JR
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Mar;26(3):448-55
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Mar;26(3):448-55
The microenvironment of human neuroblastoma supports the activation of tumor-associated T lymphocytes.
Carlson LM, De Geer A, Sveinbjørnsson B, Orrego A, Martinsson T, Kogner P, Levitskaya J
Oncoimmunology 2013 Mar 1;2(3):e23618
Oncoimmunology 2013 Mar 1;2(3):e23618
HLA Class II tetramers reveal tissue-specific regulatory T cells that suppress T-cell responses in breast carcinoma patients.
Schmidt HH, Ge Y, Hartmann FJ, Conrad H, Klug F, Nittel S, Bernhard H, Domschke C, Schuetz F, Sohn C, Beckhove P
Oncoimmunology 2013 Jun 1;2(6):e24962
Oncoimmunology 2013 Jun 1;2(6):e24962
Tissue biomarkers in melanoma patients treated with TIL.
Knol AC, Nguyen JM, Pandolfino MC, Quéreux G, Brocard A, Peuvrel L, Saint-Jean M, Saiagh S, Khammari A, Dréno B
PloS one 2012;7(12):e48729
PloS one 2012;7(12):e48729
Decrease in proportion of CD19+ CD24(hi) CD27+ B cells and impairment of their suppressive function in Graves' disease.
Zha B, Wang L, Liu X, Liu J, Chen Z, Xu J, Sheng L, Li Y, Chu Y
PloS one 2012;7(11):e49835
PloS one 2012;7(11):e49835
Paradoxically increased FOXP3+ T cells in IBD do not preferentially express the isoform of FOXP3 lacking exon 2.
Lord JD, Valliant-Saunders K, Hahn H, Thirlby RC, Ziegler SF
Digestive diseases and sciences 2012 Nov;57(11):2846-55
Digestive diseases and sciences 2012 Nov;57(11):2846-55
Phenotypic characterization of leukocytes in prenatal human dermis.
Schuster C, Vaculik C, Prior M, Fiala C, Mildner M, Eppel W, Stingl G, Elbe-Bürger A
The Journal of investigative dermatology 2012 Nov;132(11):2581-92
The Journal of investigative dermatology 2012 Nov;132(11):2581-92
Tumor-infiltrating γδ T lymphocytes predict clinical outcome in human breast cancer.
Ma C, Zhang Q, Ye J, Wang F, Zhang Y, Wevers E, Schwartz T, Hunborg P, Varvares MA, Hoft DF, Hsueh EC, Peng G
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 15;189(10):5029-36
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 15;189(10):5029-36
HTLV-1 modulates the frequency and phenotype of FoxP3+CD4+ T cells in virus-infected individuals.
Satou Y, Utsunomiya A, Tanabe J, Nakagawa M, Nosaka K, Matsuoka M
Retrovirology 2012 May 30;9:46
Retrovirology 2012 May 30;9:46
IDO and regulatory T cell support are critical for cytotoxic T lymphocyte-associated Ag-4 Ig-mediated long-term solid organ allograft survival.
Sucher R, Fischler K, Oberhuber R, Kronberger I, Margreiter C, Ollinger R, Schneeberger S, Fuchs D, Werner ER, Watschinger K, Zelger B, Tellides G, Pilat N, Pratschke J, Margreiter R, Wekerle T, Brandacher G
Journal of immunology (Baltimore, Md. : 1950) 2012 Jan 1;188(1):37-46
Journal of immunology (Baltimore, Md. : 1950) 2012 Jan 1;188(1):37-46
Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.
Hovhannisyan Z, Treatman J, Littman DR, Mayer L
Gastroenterology 2011 Mar;140(3):957-65
Gastroenterology 2011 Mar;140(3):957-65
Intracerebral human regulatory T cells: analysis of CD4+ CD25+ FOXP3+ T cells in brain lesions and cerebrospinal fluid of multiple sclerosis patients.
Fritzsching B, Haas J, König F, Kunz P, Fritzsching E, Pöschl J, Krammer PH, Brück W, Suri-Payer E, Wildemann B
PloS one 2011 Mar 18;6(3):e17988
PloS one 2011 Mar 18;6(3):e17988
The numbers of FoxP3+ lymphocytes in sentinel lymph nodes of breast cancer patients correlate with primary tumor size but not nodal status.
Gupta R, Babb JS, Singh B, Chiriboga L, Liebes L, Adams S, Demaria S
Cancer investigation 2011 Jul;29(6):419-25
Cancer investigation 2011 Jul;29(6):419-25
Immunostaining of PD-1/PD-Ls in liver tissues of patients with hepatitis and hepatocellular carcinoma.
Wang BJ, Bao JJ, Wang JZ, Wang Y, Jiang M, Xing MY, Zhang WG, Qi JY, Roggendorf M, Lu MJ, Yang DL
World journal of gastroenterology 2011 Jul 28;17(28):3322-9
World journal of gastroenterology 2011 Jul 28;17(28):3322-9
HTLV-1 bZIP factor induces T-cell lymphoma and systemic inflammation in vivo.
Satou Y, Yasunaga J, Zhao T, Yoshida M, Miyazato P, Takai K, Shimizu K, Ohshima K, Green PL, Ohkura N, Yamaguchi T, Ono M, Sakaguchi S, Matsuoka M
PLoS pathogens 2011 Feb 10;7(2):e1001274
PLoS pathogens 2011 Feb 10;7(2):e1001274
Progesterone promotes differentiation of human cord blood fetal T cells into T regulatory cells but suppresses their differentiation into Th17 cells.
Lee JH, Ulrich B, Cho J, Park J, Kim CH
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 15;187(4):1778-87
Journal of immunology (Baltimore, Md. : 1950) 2011 Aug 15;187(4):1778-87
A longitudinal, integrated, clinical, histological and mRNA profiling study of resistance exercise in myositis.
Nader GA, Dastmalchi M, Alexanderson H, Grundtman C, Gernapudi R, Esbjörnsson M, Wang Z, Rönnelid J, Hoffman EP, Nagaraju K, Lundberg IE
Molecular medicine (Cambridge, Mass.) 2010 Nov-Dec;16(11-12):455-64
Molecular medicine (Cambridge, Mass.) 2010 Nov-Dec;16(11-12):455-64
Refractory colitis following anti-CTLA4 antibody therapy: analysis of mucosal FOXP3+ T cells.
Lord JD, Hackman RC, Moklebust A, Thompson JA, Higano CS, Chielens D, Steinbach G, McDonald GB
Digestive diseases and sciences 2010 May;55(5):1396-405
Digestive diseases and sciences 2010 May;55(5):1396-405
Recipient NOD2/CARD15 status affects cellular infiltrates in human intestinal graft-versus-host disease.
Landfried K, Bataille F, Rogler G, Brenmoehl J, Kosovac K, Wolff D, Hilgendorf I, Hahn J, Edinger M, Hoffmann P, Obermeier F, Schoelmerich J, Andreesen R, Holler E
Clinical and experimental immunology 2010 Jan;159(1):87-92
Clinical and experimental immunology 2010 Jan;159(1):87-92
Inducing CTLA-4-dependent immune regulation by selective CD28 blockade promotes regulatory T cells in organ transplantation.
Poirier N, Azimzadeh AM, Zhang T, Dilek N, Mary C, Nguyen B, Tillou X, Wu G, Reneaudin K, Hervouet J, Martinet B, Coulon F, Allain-Launay E, Karam G, Soulillou JP, Pierson RN 3rd, Blancho G, Vanhove B
Science translational medicine 2010 Feb 3;2(17):17ra10
Science translational medicine 2010 Feb 3;2(17):17ra10
CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.
Green AM, Mattila JT, Bigbee CL, Bongers KS, Lin PL, Flynn JL
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
The Journal of infectious diseases 2010 Aug 15;202(4):533-41
Increased quantity of tumor-infiltrating FOXP3-positive regulatory T cells is an independent predictor for improved clinical outcome in extranodal NK/T-cell lymphoma.
Kim WY, Jeon YK, Kim TM, Kim JE, Kim YA, Lee SH, Kim DW, Heo DS, Kim CW
Annals of oncology : official journal of the European Society for Medical Oncology 2009 Oct;20(10):1688-96
Annals of oncology : official journal of the European Society for Medical Oncology 2009 Oct;20(10):1688-96
Foxp3 regulates megakaryopoiesis and platelet function.
Bernard JJ, Seweryniak KE, Koniski AD, Spinelli SL, Blumberg N, Francis CW, Taubman MB, Palis J, Phipps RP
Arteriosclerosis, thrombosis, and vascular biology 2009 Nov;29(11):1874-82
Arteriosclerosis, thrombosis, and vascular biology 2009 Nov;29(11):1874-82
Early life exposure to lipopolysaccharide suppresses experimental autoimmune encephalomyelitis by promoting tolerogenic dendritic cells and regulatory T cells.
Ellestad KK, Tsutsui S, Noorbakhsh F, Warren KG, Yong VW, Pittman QJ, Power C
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 1;183(1):298-309
Journal of immunology (Baltimore, Md. : 1950) 2009 Jul 1;183(1):298-309
CD49d provides access to "untouched" human Foxp3+ Treg free of contaminating effector cells.
Kleinewietfeld M, Starke M, Di Mitri D, Borsellino G, Battistini L, Rötzschke O, Falk K
Blood 2009 Jan 22;113(4):827-36
Blood 2009 Jan 22;113(4):827-36
The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells.
Law JP, Hirschkorn DF, Owen RE, Biswas HH, Norris PJ, Lanteri MC
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009 Dec;75(12):1040-50
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2009 Dec;75(12):1040-50
Regulatory T cells and the PD-L1/PD-1 pathway mediate immune suppression in malignant human brain tumors.
Jacobs JF, Idema AJ, Bol KF, Nierkens S, Grauer OM, Wesseling P, Grotenhuis JA, Hoogerbrugge PM, de Vries IJ, Adema GJ
Neuro-oncology 2009 Aug;11(4):394-402
Neuro-oncology 2009 Aug;11(4):394-402
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, Haase AT
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, Haase AT
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6798-807
Malignant Tregs express low molecular splice forms of FOXP3 in Sézary syndrome.
Krejsgaard T, Gjerdrum LM, Ralfkiaer E, Lauenborg B, Eriksen KW, Mathiesen AM, Bovin LF, Gniadecki R, Geisler C, Ryder LP, Zhang Q, Wasik MA, Odum N, Woetmann A
Leukemia 2008 Dec;22(12):2230-9
Leukemia 2008 Dec;22(12):2230-9
Epigenetic inheritance of DNA methylation limits activation-induced expression of FOXP3 in conventional human CD25-CD4+ T cells.
Nagar M, Vernitsky H, Cohen Y, Dominissini D, Berkun Y, Rechavi G, Amariglio N, Goldstein I
International immunology 2008 Aug;20(8):1041-55
International immunology 2008 Aug;20(8):1041-55
Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes.
Kasprzycka M, Zhang Q, Witkiewicz A, Marzec M, Potoczek M, Liu X, Wang HY, Milone M, Basu S, Mauger J, Choi JK, Abrams JT, Hou JS, Rook AH, Vonderheid E, Woetmann A, Odum N, Wasik MA
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2506-12
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2506-12
Gamma c-signaling cytokines induce a regulatory T cell phenotype in malignant CD4+ T lymphocytes.
Kasprzycka M, Zhang Q, Witkiewicz A, Marzec M, Potoczek M, Liu X, Wang HY, Milone M, Basu S, Mauger J, Choi JK, Abrams JT, Hou JS, Rook AH, Vonderheid E, Woetmann A, Odum N, Wasik MA
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2506-12
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2506-12
Foxp3 expression in human cancer cells.
Karanikas V, Speletas M, Zamanakou M, Kalala F, Loules G, Kerenidi T, Barda AK, Gourgoulianis KI, Germenis AE
Journal of translational medicine 2008 Apr 22;6:19
Journal of translational medicine 2008 Apr 22;6:19
IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.
Ahmadzadeh M, Rosenberg SA
Blood 2006 Mar 15;107(6):2409-14
Blood 2006 Mar 15;107(6):2409-14
Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level.
Roncador G, Brown PJ, Maestre L, Hue S, Martínez-Torrecuadrada JL, Ling KL, Pratap S, Toms C, Fox BC, Cerundolo V, Powrie F, Banham AH
European journal of immunology 2005 Jun;35(6):1681-91
European journal of immunology 2005 Jun;35(6):1681-91
Outcome in Hodgkin's lymphoma can be predicted from the presence of accompanying cytotoxic and regulatory T cells.
Alvaro T, Lejeune M, Salvadó MT, Bosch R, García JF, Jaén J, Banham AH, Roncador G, Montalbán C, Piris MA
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Feb 15;11(4):1467-73
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Feb 15;11(4):1467-73
FOXP3, a selective marker for a subset of adult T-cell leukaemia/lymphoma.
Roncador G, Garcia JF, Garcia JF, Maestre L, Lucas E, Menarguez J, Ohshima K, Nakamura S, Banham AH, Piris MA
Leukemia 2005 Dec;19(12):2247-53
Leukemia 2005 Dec;19(12):2247-53
The expression of the regulatory T cell-specific forkhead box transcription factor FoxP3 is associated with poor prognosis in ovarian cancer.
Wolf D, Wolf AM, Rumpold H, Fiegl H, Zeimet AG, Muller-Holzner E, Deibl M, Gastl G, Gunsilius E, Marth C
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Dec 1;11(23):8326-31
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Dec 1;11(23):8326-31
CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene.
Takahata Y, Nomura A, Takada H, Ohga S, Furuno K, Hikino S, Nakayama H, Sakaguchi S, Hara T
Experimental hematology 2004 Jul;32(7):622-9
Experimental hematology 2004 Jul;32(7):622-9
The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery.
Sakaguchi S
The Journal of clinical investigation 2003 Nov;112(9):1310-2
The Journal of clinical investigation 2003 Nov;112(9):1310-2
Control of regulatory T cell development by the transcription factor Foxp3.
Hori S, Nomura T, Sakaguchi S
Science (New York, N.Y.) 2003 Feb 14;299(5609):1057-61
Science (New York, N.Y.) 2003 Feb 14;299(5609):1057-61
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- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 5 µg/mL Mouse IgG1 Isotype Control Purified (left) or 5 µg/mL Anti-Human Foxp3 Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin-HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.
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- Figure 1 T FR expansion in human lymph nodes (LNs) during HIV infection. ( a ) Representative images of immunofluorescently stained LN sections from a subject with chronic, untreated HIV infection ( n =17) and an uninfected control subject ( n =8). LNs were stained with fluorescently labelled antibodies to CD20 (white), IgD (blue), Foxp3 (green) and CD4 (red). Follicle (F) was defined as CD20 + (white line) and germinal centre (GC) was defined as CD20 + IgD - (yellow line). Images were scanned at x 60 magnification and scale bars equal 20 mum. ( b ) Foxp3 + CD4 + cells were quantified in different regions of immunofluorescently stained LN shown in a from uninfected ( n =8) and HIV-infected ( n =17) subjects using visual inspection and quantitative image analysis to determine areas. ( c ) The average areas of total (LN), follicular and GC regions per LN cross-section were determined by quantitative image analysis. ( d ) The average number of CD4 + Foxp3 + cells per LN, F and GC cross-section was calculated by multiplying the frequency of CD4 + Foxp3 + cells per mm 2 ( b ) by the average area of each region ( c ) for each subject. The horizontal bars of each graph indicate the median value and are listed where appropriate for clarity. Statistical analyses were performed by Mann-Whitney (Wilcoxon) tests to compare unpaired, nonparametric values and significance is denoted by asterisks where * P
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- Figure 2. Expression of CD25 and FOXP3 in peripheral blood lymphocytes and tumor-associated lymphocytes from neuroblastoma patients. ( A ) Flow cytometry data demonstrate the expression of CD25 on both peripheral blood lymphocytes (PBLs) and tumor-associated lymphocytes (TALs) on the day of tumor excision in different CD3 + T-cell subsets. Gray bars represent PBLs and black bars represent TALs. One representative pattern of cell-surface CD25 expression in PBLs and TALs from patient 6 as detected by flow cytometry on the day of tumor excision is shown. Numbers indicate the percent of cells expressing or not expressing CD25 among CD4 + cells. ( B ) Expression of FOXP3 in CD4 + PBLs (gray bars) and CD4 + TALs (black bars) on the day of tumor excision, as analyzed by flow cytometry. One representative pattern of FOXP3 expression in PBLs and TALs of patient 6 is shown. Number indicates the percentage of positive cells. * = not performed.
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- Figure 3. Tetramer staining of conventional and regulatory T cells from breast cancer patients. ( A-G ) Peripheral blood mononuclear cells (PBMCs) of a breast cancer patient were analyzed, upon gating on living CD3 + CD4 + T cells. The patient sample was stained with tetramers presenting either mam 34-48 ( B ) or the CLIP peptide ( C ). Numbers indicate the percentage of cells in the respective gate, referring to lymphocytes ( A ) or CD3 + CD4 + T cells ( B ) and ( C ). Within CD3 + CD4 + T cells, regulatory T cells (Tregs) were identified as CD25 high CD127 low ( D ) and ( G ) and CD25 high CD127 low FOXP3 + ( E ), ( F ) and ( H ). ( G ) reports the percentage of Tregs within tetramer-positive cells in a representative patient. ( I ) Frequency of mam 34-48 - and CLIP-presenting tetramer-positive cells among CD3 + CD4 + T cells of breast cancer patients and healthy donors (HD) (p values as per Mann-Whitney U tests are indicated). ( J and K ) Frequency of mam 34-48 -specific Tcon ( J ) cells and Tregs ( K ) of breast cancer patients and HDs.
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- Fig. 1 PegINFalpha increases Treg and highly suppressive Treg cells in peripheral blood of PV and ET patients. PBMC were collected from patients with PV or ET treated with PegINFalpha or HU for at least 70 days (range 70-616, median 112 for PegINFalpha and range 113-2422, median 175 for HU treated patients). Lymphocytes were analyzed by flow cytometry using surface markers, CD3, CD4, CD25, CD39, HLA-DR and intracellular markers Foxp3, Ki-67 and Helios. a represents CD4 + CD25 + Foxp3 + Treg cells. In b the frequency of Treg was analyzed at different time points after initiation of PegINFalpha treatment. c-e show frequency of Ki-67 + Treg, Helios + Treg, and CD39 + /HLA-DR + ) Treg
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- Fig. 1 Ipilimumab downregulates cell CTLA-4. a CHO cells stably expressing human CTLA-4 were treated with either control human IgG or Ipilimumab (Ipi) for 4 h at 37 degC, then the CTLA-4 protein levels were analyzed by western blot. b Cell surface CTLA-4 on CHO cells used in ( a ) was measured by flow cytometry. To avoid the caveat associated with CTLA-4 masking by residual antibodies from the treatment phase, the cells were incubated with Ipilimumab (10 mug/mL) for 30 min at 4 degC before the staining with the non-competing anti-CTLA-4 antibody, clone BNI3. c Plasma membrane and cytosolic proteins purified from ( b ) were detected for CTLA-4 by immunoblot. Na + -K + ATPase and Tubulin were used as controls for purity of cellular fractionation. d Ten-days old Ctla4 h/h mice (body weight: 4.5-5.3 g; n = 6) were treated with anti-mouse PD-1 antibody intraperitoneally (i.p.) (100 mug/mouse). 24 h later, mice were further treated (i.p.) with 100 mug of control hIgG Fc or Ipilimumab for 4 h. e , f Cell surface ( e ) and total CTLA-4 ( f ) in Tregs isolated from spleen or lung were evaluated by flow cytometry. g , h Human PBMCs from healthy donors were stimulated with anti-CD3/anti-CD28 for 2 days and then treated with either control hIgGFc or Ipilimumab for 4 h at 37 degC. Cell surface CTLA-4 on CD4 + CD25 + FOXP3 + Tregs, CD4 + CD25 + FOXP3 - non-Tregs and CD8 + CD25 + cells were measured by flow cytometry. Again, for all the flow cytometry data of CTLA-4-positive cells treated w
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- Figure 2 Representative micrographs of CD3, CD8, CD45RO, CD68 IHC (x10) as well as FoxP3 and PD-L1 IHC (x20) in pediatric and adult tonsils, respectively, from the bridging study.
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- Figure 3 DIA results from IHC. Representative IHC stained images and their corresponding DIA segmentation overlays of CD3, CD8, CD45RO, CD68, FoxP3, and PD-L1 IHC (x20) from ganglioneuroblastoma, the indication showing the highest PD-L1 expression, far left, and from nephroblastoma, the indication showing the lowest PD-L1 expression, far right. Same cases and similar regions from serial sections were selected. Classification colors indicate blue for negative nuclei, brown for CD3, CD8, CD45RO, CD68, and FoxP3 positive cells; and yellow, orange, and red for PD-L1 positive cells of increasing stain intensity. The boxplots in the middle panel show distribution profiles of investigated biomarkers across investigated indications. Indications are sorted by descending order of mean PD-L1 positive cell densities. Horizontal lines mark indication pairs where the differences in distribution are statistically significant and corresponding adjusted P values are indicated above.
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- FIGURE 5 Flow cytometry revealed central memory T cell, Th1, Th17, Th22 and Treg proportions increased in PBMCs derived from CP/CPPS patients than healthy controls. PBMCs were incubated with various fluorescein-labelled antigens for surface staining. PerCP/Cyanine5.5-conjugated CD3 and FITC-conjugated CD4 were used for the central memory T cell, Th1, Th17 and Th22 cell staining, and PE-conjugated CD25 and FITC-conjugated CD4 were incubated for staining Treg cells. After fixing and permeabilizing with cell fixation/permeabilization kit, (A) for samples staining central memory T cells were incubated with PE-conjugated CD45RA and APC-conjugated CD62L; (C) for samples staining, Th1 cells were incubated with PE-conjugated IFN-gamma; (E) for samples staining, Treg cells were incubated with eFluor 660-conjugated FoxP3; (G) for samples staining, Th17 cells were incubated with PE-conjugated IL-17A; and (I) for samples staining, Th22 cells were incubated with PE-conjugated IL-17A and APC-conjugated IL-22. The quantification data were presented in B, D, F, H and J. * P < 0.05; PBMC, peripheral blood mononuclear cell
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- Figure 5. Immune and stromal cells survive in slice culture. IHC for (A) T cell markers (CD3, CD8 + , and FOXP3) (N = 3) and (B) macrophage markers (CD68, HLA-DR and CD163) was performed on days 1 and 6 of PDA slice cultures (all Bar = 50 mum). (N = 3) (C) Quantitation of cells staining for each marker demonstrates stability of immune cell populations. (N = 3) Error bars represent STDEV. (D) Day 1 and 6 slices were stained with alpha smooth muscle actin (alphaSMA) (Bar = 100 mum). (N = 4) The right panels are the expanded views of the region of interest indicated by the black boxes in the left panels.
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- Figure 1 Immunohistochemistry (IHC) of CD3, CD8, and FoxP3 T lymphocytes in CRC. Representative IHC showing low and high CD3 + , CD8 + , and FoxP3 + T lymphocytes densities. Magnification: 200x.
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- Figure 3 The MPNST immune landscape is determined by genetic background. ( A ) Levels of CD8+ T cells in terminally-harvested MPNSTs are similar across all host strains. ( B ) Infiltration of CD4+ T cells are significantly lower in tumors from C57BL/6 mice compared to MPNSTs in mice from 129X1, BALB/c, and 129/SvJae backgrounds. ( C ) Foxp3+ Tregs are detected at higher levels in tumors from 129X1 mice compared to C57BL/6 and BALB/c mice. ( D ) MPNSTs from BALB/c mice have significantly higher levels of infiltrating F4/80+ macrophages compared to C57BL/6 mice. ( E ) Mast cell infiltration is higher in tumors from BALB/c mice compared to 129/SvJae, C57BL/6, and 129X1 mice. Mast cell levels are lowest in MPNSTs from C57BL/6 mice. 129X1 (red circles), C57BL/6 (black triangles), BABL/c (white squares), and 129/SvJae (blue triangles). Analyzed by one-way ANOVA with Tukey's multiple comparison test. A p -value of less than 0.05 is considered statistically significant and is denoted by ""*"" ( n = 5 tumors per strain).
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- FIGURE 9 Results of positive correlation analysis. In this study, we analyze two pairs of markers: CD3+, CD8+ and CD4+, FoxP3+. Each marker within the correlation pair is defined as a ""channel"". (A) DoC scores distributions for Cases 1A (left island) and 1B (right island). (B) Histograms of CD3+ and CD8+ DoC scores for Case 1A (left) and 1B (right). Red line is the predetermined threshold to select potential correlated cells. (C) Selected cells for both channels are mapped for clustering analysis (left) using HDBSCAN algorithm. We then define those clusters that both channels are involved as correlation hotspots (right, each cluster is represented by a colored outline). Green: normal tissue (N); Red: invasive front (IF); Yellow: central tumor (CT). (D) Statistical analysis. Top row: proportions of correlated CD3+ and CD8+ cells and density of correlated hotspots in different regions; bottom row: same metrics for CD4+ and FoxP3+.
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- Figure 5. Flow cytometry analysis of HDAC9 expression by different cell subsets from patients with AITD. Peripheral blood mononuclear cells from patients with AITD and healthy controls were immunostained for CD3 + (T lymphocytes), CD4 + CD25 + Foxp3 + (T regulatory cells), CD19 + (B lymphocytes), CD14 + (monocytes) and HLA-DR + CD11c + BDCA1 + (myeloid dendritic cells), and HDAC9, and the cells were analyzed by flow cytometry, as stated in ""Methods."" Analysis strategy is shown in the dot plot left panel. A significant increase in the percent of HDAC9 + Treg (CD4 + CD25 + Foxp3 + ) cells was detected in patients with AITD compared with healthy controls. (right panel). * P < 0.05; **** P < 0.001.
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- Figure 4 Single vs. triple stainings, immunohistochemistry on tonsil sections. ( A - C ) Single stainings: ( A ) Bcl6 red after 15 min TRIS pH 9, ( B ) FoxP3 brown after 20 min TRIS pH 6.1, ( C ) PAX5 blue after 20 min TRIS pH 6.1. ( D ) Human tonsil with triple staining IHC, according to the FOLGAS protocol: Bcl6 red, FoxP3 brown, PAX5 blue; antigen retrievals equivalent to the corresponding single staining. Magnifications indicated by bar. ( E ) Sum of H-scores of the respective stainings under single and triple staining conditions, from two independent observers.
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- Fig. 4 CP DCL cells inhibit the allogeneic immune rejection of the same hESC line-derived cardiomyocytes in Hu-mice. (a) Schematic description of the experiments. DCL cells derived from WT and CP hESCs were delivered via the tail vein into Hu-mice. The experiments were performed using 2 different hESCs lines, H1 and HUES3. Seven days after intravenous injection of DCLs, the same hESC line-derived cardiomyocytes were transplanted into the skeletal muscle of Hu-mice and NSG control mice. (b) Cardiomyocyte survival and T cell infiltration into the cardiomyocyte graft 2 weeks after transplantation ( n = 5, H1 n = 3, HUES3 n = 2). (c) Cardiomyocyte survival and T cell infiltration into the cardiomyocyte graft 4 weeks after transplantation ( n = 4, H1 n = 2, HUES3 n = 2). Sections were stained with anti-CD3 (shown in green) and anti-CTnI (shown in red) antibodies. For each section, the numbers of CD3 + T cells and CTnI cells were counted, 3 sections per graft were analyzed. The graft survival rate was calculated in comparison to the graft survival in NSG mice. The T cell infiltration rate was calculated in comparison to that of grafts in Hu-mice without any DCL cell injection. Data are presented as mean values +- SEM. Data from different groups were compared using one-way ANOVA test. * P
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- Fig. 5 CP DCL cells inhibit the allogeneic immune rejection of the same hESC line-derived smooth muscle cells in Hu-mice. (a) Characterization of hESC-derived smooth muscle cells (SMCs). The expression of SM22 was analyzed by Flow cytometry and immunofluorescence microscopy, scale bar 50 mum. (b) SMC survival and T cell infiltration in the grafts 2 weeks after transplantation. Seven days after intravenous injection of DCLs, the same hESC line-derived SMCs were transplanted into the skeletal muscle of Hu-mice and NSG control mice ( n = 5). Data are presented as mean values +- SEM. Data from different groups were compared using one-way ANOVA test. ** p
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- Fig. 5 The immunomodulatory role of anti-RANKL in BC. a Change from baseline in serum levels of free-sRANKL ( n = 23, p = 2.384e-07) and CTX ( n = 17, p = 1.526e-05) (significance assessed by the two-tailed sign test), the percentage of Ki-67-positive cells ( p = 0.485) and the staining of activated caspase-3 ( p = 0.391) (H-score) ( n = 24) (significance assessed by two-tailed paired t -tests). Boxplots display median line, IQR boxes, 1.5 x IQR whiskers, and data points. b Each bar plot shows the change from baseline (Delta; post- minus pretreatment values) of the immune parameters assessed using HE (TILs) and IHC (CD3, CD20, CD8, FOXP3, proliferative TILs (TILs Ki67+ ), CD68, and CD163). Each bar represents one patient, which are ranked by their increase in stromal TIL levels. Geometric mean changes, 95% CIs, and p -values are shown below each bar plot. For each measured parameter, the corresponding boxplot is displayed on the right-hand side. Boxplots display median line, IQR boxes, 1.5 x IQR whiskers, and data points. Tumor characteristics and tumor RANK metagene expression at baseline are shown above. p; p -values derived from two-tailed paired t -tests (* p < 0.05) # . c Representative micrographs of multiplex IHC of pre- and posttreatment tumor sections from the four patients with the highest immunomodulatory response. White scale bar, 100 mum. d Top 20 significantly enriched pathways after denosumab treatment, identified by GAGE. e Comparison of baseline serum levels
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- Figure 3 NPC tumor-released exosomes inhibited Th1 and Th17 cell induction but promoted Tregs by altering STAT protein phosphorylation A. Differentiation of naive CD4 + T cells into Th1 and Th17 cells and Tregs after stimulation with tumor cells with or without EXOs. Purified naive CD4 + T cells from healthy donors were co-cultured with the irradiated NPC cell line TW03 (EBV + ) and then treated with EXOs or left or untreated in the presence of OKT3 in IL-2 medium for 5 days. INFgamma, IL-17, and Foxp3 staining following by FACS analysis was performed after stimulation with PMA/ionomycin. Representative data of five experiments are shown. B. Numerous data showing the mean percentage +- S.E.M. of positive cell subsets in three independent experiments. * means P < 0.05. C. Western blot analysis for the expression of P-STAT1, P-STAT3, and P-STAT5 proteins. Stimulation with EXOs decreased the expression of P-STAT1 and P-STAT3 but increased the expression of P-STAT5 in OKT3 and NPC cells stimulated by CD4 + T cells; the GAPDH gene was included as a control. Representative data of three experiments are shown.
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- Figure 3 HIV entry and replication promote T FR expansion. Disaggregated tonsil cells were spinoculated with X4 or R5 HIV and T FR populations were analysed by flow cytometry ( n =15). ( a ) A representative example of tonsil cell flow gating. From viable CD3 + CD8 - cells, T FR are defined as CXCR5 + and CD25 hi CD127 - . T FR cells contain Foxp3 + cells, whereas remaining T FH (CXCR5 + CD25 lo/- ) cells are Foxp3 - . ( b ) Percentages of T FR determined by gating strategies in a are shown. Experimental conditions include mock-spinoculated cells cultured with PMA (50 ng ml -1 ) and ionomycin (1 mug ml -1 ) or exogenous TGF-beta (100 ng ml -1 ) for 24 h and cells pretreated to block CXCR4 (AMD, 200 muM) and CCR5 (MVC, 2 muM). ( c ) Using flow cytometry counting beads, the number of cells per mul were determined for total (CD3 + CD8 - ), T FH (CXCR5 + CD25 lo/- ) and T FR (CXCR5 + CD25 hi CD127 - ) subsets in mock- and X4-spinoculated samples ( n =3). ( d ) Bcl-6 expression is shown in CXCR5- (grey), T FH (blue) and T FR (red) populations after mock-, X4- or R5-spinoculation ( n =5). ( e ) Blimp-1 expression was also determined as in d . The horizontal bars of each graph indicate the median value and are listed where appropriate for clarity. Statistical analyses were performed by Friedman nonparametric tests ( b , d , e ) and significance is denoted by asterisks where * P
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- Figure 3 Naive CD4 + T cells are converted to functional Tregs by tumor-infiltrating DCs and tumor conditioned medium (CM). (A-C) Naive CD4 + T cells from peripheral blood of patients with invasive breast carcinoma were co-cultured with or without autologous pDCs isolated from tumor (TI) or peripheral blood (PB) for 9 days in the presence or absence of 30% CM from autologous tumor slices or adjacent normal tissue slices. (A , B) Non-adherent cells from co-cultures were stained for CD3, CD4, CD25 and intracellular Foxp3, and analyzed by flow cytometry. Representative plots of gated CD3 + CD4 + cells (A) and quantification of percentage of Foxp3 + CD25 + cells among CD3 + CD4 + cells (B) are shown (mean +- SEM, n = 19; * P < 0.05, ** P < 0.01, *** P < 0.001 by Student's t -test). (C) Expression of Treg-associated genes, assessed by qRT-PCR normalized to GAPDH , in sorted CD4 + T cells, relative to expression in cultures without DCs or CM (mean +- SEM, n = 19; * P < 0.05, ** P < 0.01, *** P < 0.001 compared with naive CD4 + T cells cultured alone by Student's t -test). (D-G) Effect of naive CD4 + T cell-derived Tregs, obtained by co-culture with TI pDCs and tumor CM as above, on function of autologous tumor-specific CD8 + T cells. Tumor-specific CD8 + T cells were generated for each subject by stimulating autologous PB CD8 + T cells with autologous tumor lysate-pulsed autologous DCs. Tregs were recovered from co-cultures by magnetic sorting. (D) CFSE-labeled CD8 + T ce
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- Figure 6 In vivo knockdown of PITPNM3 in CD4 + T cells reverses immunosuppression and inhibits tumor progression in humanized mice. (A) Humanized mice bearing palpable MDA-MB-231 orthotopic xenografts were intraperitoneally injected daily for 14 days with PBS, 1 nmol CD4-aptamer-control siRNA (AsiC-con) or CD4-aptamer-siRNA targeting PITPNM3 (sequence in A , AsiC-PI) to assess the role of PITPNM3 in TI Tregs, and other T cells and tumor control. Experimental schematic is provided in Supplementary information, Figure S9A . (B) Representative immunoblots showing selective knockdown of PITPNM3 protein in PB CD4 + T cells, but not tumor xenografts ( n = 3). (C) PITPNM3 knockdown did not affect the distribution of human CD45 + hematopoietic cells, CD4 + and CD8 + T cells, and CD14 + monocytes in the peripheral blood of humanized mice. Representative flow plots are shown ( n = 3). (D , E) Effect of PITPNM3 knockdown on TI naive CD4 + , Tregs and CD8 + T cell numbers, and apoptosis by TUNEL assay in xenografts. D shows representative immunofluorescence microscopy images. Top row indicates CD4 + naive T cells by arrows; the second row indicates CD4 + CD45RO + Foxp3 - CD4 + memory T cells (yellow arrows) and Foxp3 + Tregs (white arrows). Scale bar, 50 mum. E shows number of cells of each subtype/high power field in eight mice ( ** P < 0.01, *** P < 0.001 compared to PBS group by Student's t -test). (F) Flow cytometry analysis of gated human CD3 + CD4 + cells isolated from xenogra
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- Figure 1 Multiplex IHC on tissue before and after sepsis. (A) Images showing T cell infiltration and macrophage infiltration in tissue before (omentum biopsy) and after (tumor resection) sepsis. CKAE1/AE3 was used to visualize tumor cells. Effector T cells were characterized as CD3 + CD8 + , regulatory T cells as CD3 + Foxp3 + , M1 macrophages as CD68 + , and M2 macrophages as CD163 + . (B) CD8 T cells, T Regs , M1 and M2 macrophages per mm 2 . Immune cells were quantified in control tissue, and in tissue obtained before and after sepsis. (C) CD8 effector T cell/T Reg ratio and M1/M2 ratio before and after sepsis. (D) Average staining intensity of immune cell markers before and after sepsis. Unstained tissue was used as control. Untreated primary tumor from stage III and IV ovarian cancer patient were used as control ( n = 16). Red line indicates mean.
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- Figure 1. Spectrum of tumor-infiltrating lymphocytes characterized by MIBC PD-L1 staining. (A) Normalized histograms are shown with the TIL densities (TIL counts per 100 tumors cells) binned into the designated intervals on the x -axes and the sample proportions shown on the y -axes. In each case (overall, CD8, and FOXP3 + TILs) a significant (*Goodman-Kruskal p < 0.01) increase in the amount of TILs is observed with increased MIBC PD-L1 staining. (B) The percentage of cases with MIBC PD-L1 positivity (defined as > 0%) is shown on the x -axes and the per sample CD8/Treg ratio on the y -axes. No statistically significant (NS) association to MIBC PD-L1 expression status was observed.
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- Figure 6 FoxP3 and Helios expression in CD4 + T cells PBMC from HD and PBC patients, NILs and TILs were stained for CD3 and CD4 antibodies followed by intracellular staining for FoxP3 and Helios. Live cells were first gated using Fixable Viability Dye 660. ( A ). Representative flow cytometric plots of FoxP3 staining from one cancer patient are shown. ( B ). Scatter plot showing the differences in frequencies of FoxP3 + Tregs between different samples. ( C ). IHC staining of FoxP3 + expression in one NT and TT samples. ( D ). Flow cytometric plots of FoxP3 and Helios co-expression in CD4 + T cells from different samples and whisker plots ( E ) showing differences in various FoxP3 and Helios-expressing Treg subsets. ( F ). Non-parametric Spearman's test showing correlations between FoxP3 and Helios expressions in NILs and TILs. ( G ). Pie charts show the relative percentages of different FoxP3 and Helios Treg subsets.
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- Fig. 6 Major overlap between Treg and Tconv subsets in breast tumors. a Representative immunostaining of FFPE breast tumor tissue (TT) from n = 3 biologically independent replicates. CD4 (red), CD127 (purple), FOXP3 (green), DAPI (blue). TT Treg (CD4 + FOXP3 + CD127 - ), TT Tconv (CD4 + FOXP3 - CD127 + ), TT ActTconv (CD4 + FOXP3 + CD127 + ). Scale bar denotes 2.32 um. b Pie chart of all clonotypes (unique TCRbeta nucleotide sequences) detected among TT Treg (red), TT Tconv (green), and TT ActTconv (orange) in order of decreasing percentage (%). Gray slices correspond to the TCRbeta repertoire fraction that contains clonotypes with a frequency below 1%. Representative data from breast cancer patient with mammary gland adenocarcinoma MaCa 4554 from n = 3 biologically independent replicates. c Venn diagram of shared clonotypes (white) between TT Treg, TT Tconv, and TT ActTconv within each patient. d Frequency of common clonotypes between TT Treg, TT Tconv, and TT ActTconv in relation to the frequency distribution of all clonotypes per subset. N , total number of clonotypes recovered from TT Treg (red), TT Tconv (green), and TT ActTconv (orange). e Total frequency of Treg clonotypes overlapping with Tconv among Tconv (green), with Tconv and ActTconv among Treg (red) or with ActTconv among ActTconv (orange) either in peripheral blood (PB) or within TT in n = 3 MaCa patients and n = 3 healthy individuals (HD) (cumulative data, mean +- s.e.m.). n refers to biologically independent